| 宫立众,孙士红,程天民,粟永萍,冉新泽,罗成基,郭朝华.外周血干细胞和骨髓基质细胞联合输注对急性放射病造血重建作用的实验研究[J].中华放射医学与防护杂志,2002,22(4):266-268 |
| 外周血干细胞和骨髓基质细胞联合输注对急性放射病造血重建作用的实验研究 |
| The effect of coinfusion of peripheral blood stem cells and stromal cells on hematopoietic reconstitution in lethally irradiated mice |
| 投稿时间：2001-10-26 |
| DOI： |
| 中文关键词: 外周血干细胞移植 基质细胞 辐射损伤 |
| 英文关键词:Peripheral blood stem cells Stromal cells Irradiation injury |
| 基金项目:国家重点基础研究发展规划项目(“973”项目)基金资助项目(G1999054205) |
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| 中文摘要: |
| 目的 观察外周血干细胞联合骨髓基质细胞输注对急性放射病造血功能恢复的影响。方法 8.5Gyγ射线照射的BALBc小鼠,分别输入红细胞(对照组)、动员的外周血干细胞(PB-SCT组)、外周血干细胞联合扩增的骨髓基质细胞(实验组),观察受体鼠4周的生存率、骨髓有核细胞(BMNC)、粒细胞巨噬细胞集落形成单位(CFU-GM)、成纤维细胞集落形成单位(CFU-F)、外周血白细胞计数(WBC)等指标。结果 实验组的生存率、BMNC、CFU-GM、CFU-F较对照组和PBSCT组显着升高,P<0.05;WBC的最低值较其他两组高(P<0.01),WBC恢复较PBSCT组快(P<0.01)。结论 外周血干细胞联合骨髓基质细胞输注有促进急性放射病造血重建的作用,其作用优于单纯的外周血干细胞输注(移植). |
| 英文摘要: |
| Objective To study the effect of coinfusion of peripheral blood stem cells and stromal cells on hematopoietic reconstitution in lethally irradiated mice. Methods BALB/c mice irradiated with 60 Co γ-rays were treated with RBC infusion (control group), peripheral blood stem cells infusion (PBSCT group), or coinfusion of peripheral blood stem cells and bone marrow stromal cells (experimental group).Survival rate within 4 weeks, white blood cell count, bone marrow nucleated cells (BMNC), granulocyte-macrophage colony-forming unit (GM-CFU), fibroblast colony-forming unit (F-CFU) were observed. Results Survival rate, BMNC, GM-CFU, F-CFU counts were significantly higher in the experimental group than those of the control andPBSCT groups (P <0. 05).The lowest number of WBC was significantly higher (P <0.01) and WBC recovery was faster (P <0.01) in the experimental group. Conclusion Coinfusion of peripheral blood stem cells and stromal cells could accelerate hematopoietic reconstitution in lethally irradiated mice and the effect is better than that of peripheral blood stem cell infusion (transplantation) only. |
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