| 薛文成,王宝勤,苑宾,等.慧星电泳用于肿瘤细胞辐射敏感性检测的研究[J].中华放射医学与防护杂志,2002,22(4):263-265.XUE Wencheng,WANG Baoqin,YUAN Bin,et al.Comet-electrophoresis assay as a method for determining radiosensitivities of tumor cells[J].Chin J Radiol Med Prot,2002,22(4):263-265 |
| 慧星电泳用于肿瘤细胞辐射敏感性检测的研究 |
| Comet-electrophoresis assay as a method for determining radiosensitivities of tumor cells |
| 投稿时间：2001-09-26 |
| DOI： |
| 中文关键词: 肿瘤细胞 辐射敏感性 放射治疗 彗星电泳 DNA损伤 |
| 英文关键词:Tumor cells Radiosensitivities Radiation therapy Comet-electrophoresis assay DNA damage |
| 基金项目:国家攀登计划(B)基金资助项目(8545011) |
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| 中文摘要: |
| 目的 探讨彗星电泳方法检测肿瘤细胞对γ射线的辐射敏感性的可行性。方法 以自行设计的图像分析系统,用彗星电泳方法检测4种人肿瘤细胞受γ射线照射后细胞初始DNA损伤,以及细胞经30min修复时DNA损伤残余率;用细胞集落存活法检测2Gyγ射线照射后细胞存活率。结果 4种肿瘤细胞初始DNA损伤与辐射敏感性无关,2Gyγ射线照射后4种细胞存活率(SF2)与细胞经30min修复后的DNA损伤残余率相关明显(r=-087)。结论 本实验方法有可能成为一种快速、准确检测肿瘤细胞内在辐射敏感性的方法。 |
| 英文摘要: |
| Objective To explore the feasibility of applying comet-electrophoresis to determining the radiosensitivity of tumor cells. Methods The residual rates of DNA damage at 30 minute after 2 Gy gamma irradiation in four human tumor cell lines (WM9839, KB, LS-T-117, PC3M) were determined with the comet assay.The cell survival fraction of tumor cell after 2 Gy gamma ray-irradiation was determined with colonogenic assay. Results There were good correlations between cell survival fraction (SF2) and residual rate of DNA damage at 30 minute after 2 Gy gamma ray-irradiation in these four human tumor cell lines, separately. Conclusion The comet-electrophoresis assay may be used as a repaid and sensitive method for determining inherent radiosensitivities of tumor cells. |
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