| 金顺子,刘树铮.X射线全身照射对免疫细胞共刺激分子表达的影响[J].中华放射医学与防护杂志,2002,22(4):256-259 |
| X射线全身照射对免疫细胞共刺激分子表达的影响 |
| Effect of whole-body X-irradiation on the expression of costimulatory molecules on immune cells |
| 投稿时间：2001-09-20 |
| DOI： |
| 中文关键词: 电离辐射 共刺激分子 CD28 CTLA-4 B7-1 B7-2 |
| 英文关键词:X-irradiation Costimulatory molecules CD28 CTLA-4 B7-1 B7-2 |
| 基金项目:国家自然科学基金资助项目(39570188) |
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| 中文摘要: |
| 目的 探讨低剂量电离辐射对免疫细胞表面分子的影响。方法 用流式细胞术FITC-CD28/PECTLA-4双染法观察了小鼠胸腺和脾细胞的CD28和CTLA-4表达变化,用免疫细胞化学(ABC)法观察了小鼠腹腔巨噬细胞B7-1和B7-2的表达变化。结果 低剂量电离辐射和较高剂量电离辐射均能增强腹腔巨噬细胞B7分子的表达;同时发现,低剂量电离辐射显着增强胸腺和脾细胞CD28的表达,而CTLA-4的表达降低,脾细胞较胸腺细胞更明显。较高剂量电离辐射显着增强胸腺和脾细胞CTLA-4的表达,同时抑制两种细胞CD28的表达,以脾细胞为更显着。结论 共刺激分子CD28和B7的表达上调可能是低剂量电离辐射免疫兴奋效应机理中的重要因素。 |
| 英文摘要: |
| Objective To study the effect of ionizing radiation on costimulatory molecules on immune cells. Methods Flow cytometry (FCM) was used to detect the changes in the expression of CD28/CTLA-4 of thymocytes and splenocytes after whole-body irradiation (WBI) with different doses of X-rays.Immunohistochemistry (IHC, ABC method) was used to detect the changes in the expression of B7-1 and B7-2 on peritoneal ma crophages after WBI with different doses of X-rays. Results The results showed that 0.075 Gy X-rays caused significant up regulation of CD28 on both thymocytes and splenocytes and down-regulation of CTLA-4 expression, which was more significant on the splenocytes.After 2 Gy irradiation the expression of CTLA-4 on both thymocytes and splenocytes was significantly up-regulated with down-regulation of CD28 expression, which was more significant on the splenocytes.The dose-effect studies showed that there was no change in the expression of CTLA-4 on the thymocytes 72 h after LDR, while 2 and 4 Gy caused apparent increase in the expression of CTLA-4(P <0.05, P <0.01).For splenocytes, LDR caused no changes in the expression of CTLA-4 4 h after irradiation, while higher doses could increase the expression of CTLA-4 significantly (P <0.05, P <0.01).The expression of B7-1 and B7-2 on peritoneal macrophages increased after both 0.075 Gy and 2 Gy X-rays.The increase in B7-2 expression preceded that of B7-1 expression after 0.075 Gy and lasted longer than after 2 Gy irradiation. Conclusion Up-regulation of expression of costimulatory molecules CD28 and B7 after whole-body X-irradiation may be an important factor involved in the enhancing effect of low dose radiation on immunity. |
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