| 孙苏平,蔡建明,项莺松,等.SDF-1的表达与造血细胞回巢关系的实验研究[J].中华放射医学与防护杂志,2002,22(3):201-203.SUN Suping,CAI Jianming,XIANG Yingsong,et al.Experinmental study on relationship between expression of SDF-1 and homing of hematopoietic cells[J].Chin J Radiol Med Prot,2002,22(3):201-203 |
| SDF-1的表达与造血细胞回巢关系的实验研究 |
| Experinmental study on relationship between expression of SDF-1 and homing of hematopoietic cells |
| 投稿时间：2001-06-29 |
| DOI： |
| 中文关键词: 异基因小鼠 骨髓移植 回巢 SDF-1 |
| 英文关键词:Allogeneic mouse Bone marrow transplantation Homing SDF-1 |
| 基金项目:国家自然科学基金资助项目(39770329,3960077) |
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| 中文摘要: |
| 目的 探讨趋化蛋白SDF-1在造血细胞回巢中的作用。方法 采用异基因小鼠骨髓细胞移植动物模型和免疫组化双染色方法,观察趋化蛋白SDF-1的表达与回巢细胞的关系。结果 骨髓内SDF-1主要表达在骨内膜旁、微血管内皮、骨细胞内和供体阳性细胞周围,与非照射组相比,照射预处理组骨髓中的SDF-1在骨细胞中、骨内膜旁有明显高表达,提示放射预处理可能促使髓内各种基质细胞分泌SDF-1,SDF-1与回巢细胞的关系密切。结论 照射预处理可能是增加骨髓基质细胞分泌SDF-1的激发因素之一,SDF-1在造血细胞回巢中有重要作用。 |
| 英文摘要: |
| Objective To investigate the role of chemoattractant SDF-1 in of homing hematopoietic cells.Methods A mouse allogeneic bone marrow transplantation model and double staining of immunohistochemistry were used in this study.Relationship between expression of chemoattractant SDF-1 and of homing hematopoietic cells was observed.Results In bone marrow,SDF-1 was mainly expressed at sites near endosteum,in microvessel endothelium,osteocyte and around donor cells.Contrast to nonirradiation group,SDF-1 was significantly expressed by osteocytes,and at sites near endosteum in the irradiation group, suggesting that pretreatment with irradiation might enhance secretion of SDF-1 by bone marrow stromal cells and SDF-1 could have a close relation with homing cells.Conclusion Pretreatment with irradiation might be one of the stimulating factors significantly increasing the level of SDF-1 produced by bone marrow stromal cells and the chemoattractant SDF-1 could play an important role in homing of hematopoietic cells. |
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