| 刘建忠,周元国,程天民,等.5.21Gy软X射线局部照射伤口后组织细胞周期时相变化的研究[J].中华放射医学与防护杂志,2002,22(3):180-183.LIU Jianzhong,ZHOU Yuanguo,CHENG Tianmin,et al.Phase-changes in cell cycle of wound tissue irradiated with 5.21 Gy soft X-rays[J].Chin J Radiol Med Prot,2002,22(3):180-183 |
| 5.21Gy软X射线局部照射伤口后组织细胞周期时相变化的研究 |
| Phase-changes in cell cycle of wound tissue irradiated with 5.21 Gy soft X-rays |
| 投稿时间：2001-11-19 |
| DOI： |
| 中文关键词: 软X射线 局部照射 创伤愈合 细胞周期 |
| 英文关键词:Soft X ray Local irradiation Wound healing Cell cycle |
| 基金项目:国家重点基础研究发展规划“973”专项经费资助项目(G1999054205) |
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| 中文摘要: |
| 目的 研究软X射线局部照射后大鼠伤口组织细胞周期的时相变化。方法 PI染色,流式细胞术(FCM)检测细胞周期；BrdU掺入后采用SABC染色检测细胞增殖。结果 在伤后3～9d,对照侧G0G1期细胞逐渐减少,S期细胞增加,9d时达到峰值；G2M期细胞增加,15d时达到峰值。照射侧G0G1期细胞增加,S期和G2M期细胞减少。在伤后12～22d,照射侧S期细胞逐渐增加,22d时达到峰值,大量的细胞停留在S期。在整个愈合过程中,G2M期细胞百分数均低于对照侧。BrdU掺入SABC染色阳性细胞主要为成纤维母细胞、血管内皮细胞以及平滑肌细胞。结论 521Gy软X射线局部照射后,细胞发生G1期阻滞和S期延长,G2M转换障碍,细胞分裂增殖受抑,创伤愈合延迟与细胞周期的紊乱有关,G1期阻滞和S期延长越严重,愈合延长时间越长。 |
| 英文摘要: |
| Objective To study the phase changes in cell cycle of wound tissue which was locally irradiated with 5.21 Gy soft X rays.Methods Flow cytometry and PI staining were used to analyze cell cycle.Cell proliferation was determined with BrdU labeling.Results During 3 9 days after irradiation,the percentage of the G0/G1 phase cells in wound of the control side decreased while the percentage of S phase cells increased and reached the highest value on day 9.The percentage of G1/M phase cells also increased,and reached its peak on day 15.The percentage of G0/G1 phase cell increased in wound of the irradiation side and was higher than that of the control wound,meanwhile the percentages of S and G2/M cells were significantly lower than those of the control wound.In the period of 12-22 days after wounding,the percentage of S phase cells increased and reached its peak value on the 22nd day.When most of cells were in S phase and arrested dramatically.Through the whole healing process,the percentage of G2/M in wound of the irradiation side was lower than that of the non irradiated wound.The BrdU positive cells were fibroblasts,endothelial cells and smooth muscle cells.Conclusion These results suggest that G1 block,S phase arrest,and switch of G2/M with suppression of mitotic activity of these cells are induced by local 5.21 Gy soft X ray irradiation.Therefore,wound healing delay is induced partly by cell cycle arrest. |
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