| 许志祥,张学光,朱剑昆,徐颖,李颖,丁天,赵仁亮,於葛华,强亦忠.Flt3配体联合其他细胞因子对受照射小鼠免疫系统的作用研究[J].中华放射医学与防护杂志,2002,22(2):86-89 |
| Flt3配体联合其他细胞因子对受照射小鼠免疫系统的作用研究 |
| Flt3 ligand accelerates the recovery of the immune system in irradiated mice |
| 投稿时间：2000-11-21 |
| DOI： |
| 中文关键词: 细胞因子 Flt3配体 免疫系统 |
| 英文关键词:Cytokines Flt3 ligand Immune system |
| 基金项目:国防科工委国防预研基金资助项目(Y5573162);国际原子能机构(IAEA)基金资助项目(CRP9025) |
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| 中文摘要: |
| 目的 研究Flt3配体(FL)在受照射小鼠免疫系统恢复中的作用。方法 采用F800血细胞自动计数仪行外周血细胞计数及白细胞分类计数,流式细胞仪分析外周血淋巴细胞表型。结果 照射前应用FL或照射后联合应用FL、IL-11、EPO和G-CSF等细胞因子,能有效地促进受照小鼠外周血白细胞和淋巴细胞的恢复,对CD8+细胞的恢复作用尤为明显,同时使CD4+/CD8+细胞比值较早恢复正常,提高受照射小鼠的生存率。结论 FL不但能促进辐射损伤机体造血功能的恢复,对免疫系统的恢复亦具有重要的作用。FL单独或与其他细胞因子联合应用,对辐射损伤动物具有明显的防护和治疗效应。 |
| 英文摘要: |
| Objective To study the immunoprotective potential of Flt3 ligand (FL) alone or in combination with other cytokines in irradiated mice.Methods The number of peripheral blood cells was counted by using F-800 apparatus and the phenotype of lymphocytes was analyzed by flow cytometry.The pathological manifestations of spleen,thymus and lymph nodes were also evaluated.Results FL used alone before irradiation or in combination with other cytokines after irradiation could stimulate the recovery of peripheral white blood cells,especially CD8+ T lymphocytes,and accelerate the recovery of ratio of CD4+/CD8+ T lymphocytes.FL reduced immune tissue damage and stimulated recovery of spleen,thymus and lymph nodes.The administration of FL to mice prior to irradiation and,to a lesser extent,after irradiation,significantly enhanced survival rate of animals.ConclusionFL can stimulate not only the recovery of hematopoiesis,but also the improvement of immune function in irradiated mice.FL alone or in combination with other cytokines exerts a distinct radio-protection effect. |
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