| 杜楠,裴雪涛,罗成基,周进民,程天民.Egr-1调控元件启动的造血因子基因转移对辐射损伤的骨髓基质细胞的保护作用[J].中华放射医学与防护杂志,2002,22(2):80-82 |
| Egr-1调控元件启动的造血因子基因转移对辐射损伤的骨髓基质细胞的保护作用 |
| Effect of expression of hematopoietic growth factors regulated by radio-inducible promoter on radiation injury of bone marrow stromal cells |
| 投稿时间：2001-01-15 |
| DOI： |
| 中文关键词: 辐射诱导元件 造血生长因子 基质细胞 |
| 英文关键词:Radiation-inducible promoter Hematopoietic growth factor stromal cells |
| 基金项目:国家自然科学基金资助项目(39900040);国家杰出青年自然科学基金资助项目(39825111) |
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| 中文摘要: |
| 目的 观察基因修饰的骨髓基质细胞对辐射的耐受作用。方法 通过辐射对CFUF形成的影响制作细胞存活曲线分析造血因子双顺反子基因修饰的基质细胞(HFCL/FG和HFCL/EFG)对辐射的耐受,采用3H-TdR掺入法验证上述结果,观察转染细胞对粒系祖细胞生长的影响。结果 HFCL/FG和HFCL/EFG细胞生存曲线的肩明显增宽,D0值增大,N和Dq值增大,3H-TdR掺入实验也验证了该结果。这种细胞对粒系祖细胞的扩增作用明显增强。结论 造血因子基因修饰的骨髓基质细胞具有抗辐射和支持造血作用。 |
| 英文摘要: |
| Objective To explore radioresistance -improving and radio-protective effects of hematopoietic growth factor genes transfected into HFCL stromal cells.Methods Radioresistance of gene-transfected cells to radiation was detected by dose survival curve and 3H-TdR incorporation rate,Colony forming assay on cultured stroma layer was examined.Results The gene-modified cells (HFCL/EFG and HFCL/FG) showed more resistant to ionizing radiation than control HFCL cells by dose survival curve, 3H-TdR incorporation rate.The transfected genes had effects of protecting hematopoiesis and radioprotection by colony forming assay.ConclusionStromal cells transduced with hematopoietic growth factor genes can improve radioprotective effects. |
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