| 张浩,毛秉智,董波,等.SCF和IL-6双基因共转染促进小鼠造血和免疫功能的恢复[J].中华放射医学与防护杂志,2002,22(2):77-79.ZHANG Hao,MAO Bingzhi,Dong Bo,et al.Stimulating recovery of hemopoietic and immunological functions by cotransferring IL-6 and SCF genes in irradiated mice[J].Chin J Radiol Med Prot,2002,22(2):77-79 |
| SCF和IL-6双基因共转染促进小鼠造血和免疫功能的恢复 |
| Stimulating recovery of hemopoietic and immunological functions by cotransferring IL-6 and SCF genes in irradiated mice |
| 投稿时间：2008-08-10 |
| DOI： |
| 中文关键词: 骨髓基质细胞 I-L6 干细胞刺激因子 |
| 英文关键词:QXMSC IL-6 SCF |
| 基金项目:国家自然科学基金资助项目(39870716) |
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| 中文摘要: |
| 目的 探讨逆转录病毒介导的IL-6和SCF双基因共转染骨髓基质细胞对辐射小鼠造血和免疫功能的影响。方法 利用动物实验观察逆转录病毒介导的含IL6和SCF双基因共转染的骨髓基质细胞对辐射小鼠外周血、骨髓有核细胞数、骨髓细胞CFUGM培养、脾脏淋巴细胞LTT、CD4/CD8等指标的影响。结果 表明转IL6和SCF基因的骨髓基质细胞可以促进辐射小鼠造血和免疫功能的恢复。结论 IL-6与SCF双基因在骨髓基质细胞中获得表达,为进一步研究转基因骨髓基质细胞对造血调控的作用奠定了基础。 |
| 英文摘要: |
| Objective To explore the hemopoietic and immunological functions of retroviral-mediated IL-6 and SCF genes cotransferred into bone marrow stromal cells in irradiated mice.Methods IL-6 and SCF cDNA were recombined with retroviral vector pLXSN by gene recombination technolgy.Number of peripheral blood and bone marrow cells,CFU-GM of bone marrow cells,LTT and CD4/CD8 ratio of spleen were also assayed in vivo.Results The bone marrow stromal cells transferred with IL- 6 and SCF genes could stimulate the recovery of hemopoietic and immunological functions in irradiated mice in vivo.ConclusionIL- 6 and SCF genes are simultaneously expressed in bone marrow stromal cells,which provide a basis for studies on hematopoietic regulation by gene-transfected bone marrow stromal cells. |
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