中华放射医学与防护杂志

赵仁,吴心芳,张华,等.中药制剂预防放射性直肠炎37例临床观察[J].中华放射医学与防护杂志,2002,22(1):29.,et al.Effects of introducing wild-type p53 gene on the radiosensitivity of SKOV-3 ovarian cancer cells[J].Chin J Radiol Med Prot,2002,22(1):29

中药制剂预防放射性直肠炎37例临床观察

Effects of introducing wild-type p53 gene on the radiosensitivity of SKOV-3 ovarian cancer cells

投稿时间：2001-09-04

DOI：

中文关键词: 卵巢肿瘤基因 p53 转染放疗敏感性

英文关键词:Ovarian tumor Gene p53 Transfection Radiosensitivity

基金项目:辽宁省自然科学基金资助项目(972252)

作者	单位
赵仁	750004 银川, 宁夏医学院附属医院放疗科
吴心芳	750004 银川, 宁夏医学院附属医院放疗科
张华	750004 银川, 宁夏医学院附属医院放疗科
折虹	750004 银川, 宁夏医学院附属医院放疗科

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中文摘要:

放射性直肠炎是盆腔放射治疗的患者常见的并发症之一, 为了减轻放射所致的肠损伤, 提高病人的生活质量, 笔者半随机分组观察了79例患者用自制的中药灌肠剂“直肠炎一号”预防放射性直肠炎, 现将其近期疗效报道如下。

英文摘要:

Objective To study the effect of wild-type p53 gene on the radiosensitivity of SKOV-3 ovarian cancer cells. Methods Recombinant eukaryotic expression vector pcDNA3 containing full-length human wild-type p53 cDNA was introduced by lipofectamine-mediated gene transfection into cultured SKOV-3 cells which had been irradiated with 2 and 4 Gy X-rays, respectively.The radiosensitivities of the tumor cells with different p53 status were studied. Results The number of colonies in the SKOV-3, SKOV-3-vect, and SKOV-3-p53 groups decreased by 18.6%, 22.9% and 44.5%, respectively with 2 Gy irradiation, and decreased by 63.6%, 64.9% and 88.9%, respectively with 4 Gy irradiation.After introduction of p53 cDNA, the cell number in S phase and the ratio of G₂/M phase of tumor cells decreased and the ratio of G₁/G-0 phase increased.The introduction of p53 gene into cells led to cell cycle arrest in G₁ phase. Conclusion Exogenous introduction of wild-type p53 cDNA into SKOV-3 ovarian cancer cells can increase their radiosensitivity.

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