| 金镇,关婷,李守柔.野生型p53基因转染卵巢癌SKOV-3细胞对放疗敏感性的影响[J].中华放射医学与防护杂志,2002,22(1):27-29.JIN Zhen,GUAN Ting,LI Shourou.Effects of introducing wild-type p53 gene on the radiosensitivity of SKOV-3 ovarian cancer cells[J].Chin J Radiol Med Prot,2002,22(1):27-29 |
| 野生型p53基因转染卵巢癌SKOV-3细胞对放疗敏感性的影响 |
| Effects of introducing wild-type p53 gene on the radiosensitivity of SKOV-3 ovarian cancer cells |
| 投稿时间：2001-04-02 |
| DOI： |
| 中文关键词: 卵巢肿瘤 基因 p53 转染 放疗敏感性 |
| 英文关键词:Ovarian tumor Gene p53 Transfection Radiosensitivity |
| 基金项目:辽宁省自然科学基金资助项目(972252) |
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| 中文摘要: |
| 目的 研究野生型p53基因转染卵巢癌SKOV-3细胞对放疗敏感性的影响。方法 用脂质体介导的转染技术, 将人野生型p53cDNA的真核表达重组质粒分别导入受不同剂量射线照射的SKOV-3培养细胞中, 观察p53不同状态下对肿瘤细胞放疗敏感性的差异。结果 SKOV-3、SKOV3vect及SKOV-3p53经2Gy照射后, 其集落形成数分别下降了18.6%、22.9%及44.5%;经4Gy照射后, 其集落形成数分别下降了63.6%、64.9%及88.9%.转染p53基因后, 肿瘤细胞S期和G2M期的比例下降, G1G0期的比例增加, p53基因的转染使卵巢癌细胞发生G1期阻滞。结论 外源性野生型p53基因的转染, 增加了卵巢癌SKOV-3细胞对放疗的敏感性。 |
| 英文摘要: |
| Objective To study the effect of wild-type p53 gene on the radiosensitivity of SKOV-3 ovarian cancer cells. Methods Recombinant eukaryotic expression vector pcDNA3 containing full-length human wild-type p53 cDNA was introduced by lipofectamine-mediated gene transfection into cultured SKOV-3 cells which had been irradiated with 2 and 4 Gy X-rays,respectively.The radiosensitivities of the tumor cells with different p53 status were studied. Results The number of colonies in the SKOV-3,SKOV-3-vect,and SKOV-3-p53 groups decreased by 18.6%,22.9% and 44.5%,respectively with 2 Gy irradiation,and decreased by 63.6%,64.9% and 88.9%,respectively with 4 Gy irradiation.After introduction of p53 cDNA,the cell number in S phase and the ratio of G-2/M phase of tumor cells decreased and the ratio of G-1/G-0 phase increased.The introduction of p53 gene into cells led to cell cycle arrest in G-1 phase. Conclusion Exogenous introduction of wild-type p53 cDNA into SKOV-3 ovarian cancer cells can increase their radiosensitivity. |
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