中华放射医学与防护杂志

贾光宏,马业新,肖建明.⁶⁰Coγ射线照射抑制平滑肌细胞增殖的MAPK及PKC途径[J].中华放射医学与防护杂志,2002,22(1):25-26.JIA Guanghong,MA Yexin,XIAO Jianming.Inhibition of MAPK and PKC pathways by ⁶⁰Co γ-radiation in cultured vascular smooth muscle cells[J].Chin J Radiol Med Prot,2002,22(1):25-26

⁶⁰Coγ射线照射抑制平滑肌细胞增殖的MAPK及PKC途径

Inhibition of MAPK and PKC pathways by ⁶⁰Co γ-radiation in cultured vascular smooth muscle cells

投稿时间：2000-07-18

DOI：

中文关键词: 辐射平滑肌细胞信号传递蛋白激酶类

英文关键词:Radiation Smooth muscle cell Signal transduction Protein kinases

基金项目:湖北省科委“九五”攻关基金资助项目(962P1101)

作者	单位
贾光宏	430030 武汉, 华中科技大学同济医学院心内科
马业新	430030 武汉, 华中科技大学同济医学院心内科
肖建明	430030 武汉, 华中科技大学同济医学院心内科

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中文摘要:

目的探讨γ射线照射抑制平滑肌细胞(SMC)增殖的信号传递途径。方法培养的动脉平滑肌细胞分别接受吸收剂量为3.5、7.0、14Gy⁶⁰Coγ射线照射后, 以³HTdR掺入法测定平滑肌细胞增殖程度, 放射活性法测定蛋白激酶C(PKC)及丝裂素活化蛋白激酶(MAPK)活性。结果7.0、14Gy⁶⁰Coγ射线能明显抑制平滑肌细胞增殖, 同时有MAPK、PKC活性明显降低。结论 7.0、14Gy⁶⁰Coγ射线抑制血管平滑肌细胞增殖可能是通过降低MAPK、PKC活性途径实现的。

英文摘要:

Objective To investigate the signal transduction pathways inhibited by ⁶⁰ Co γ-radiation in cultured vascular smooth muscle cells (VSMC). Methods The cultured VSMC were irradiated with ⁶⁰ Co γ-radiation of 3.5,7.0,and 14 Gy respectively.VSMC proliferation was measured by 34⁺³H-TdR incorporation,while PKC,MAPK activities were determined by radioactivity assay. Results Proliferation of VSMC was inhibited by 7.0,14 Gy ⁶⁰ Co γ-irradiation and the activities of PKC,MAPK were decreased significantly. Conclusion Inhibitory effect of 7.0,14 Gy ^{60 Co γ-irradiation on proliferation of VSMC might be resulted from decrease of the activity of PKC,MAPK.}

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