| 彭瑞云,王德文,高亚兵,等.大鼠放射复合伤伤口中血管再生及内皮细胞凋亡研究[J].中华放射医学与防护杂志,2002,22(1):20-21.PENG Ruiyun,WANG Dewen,GAO Yabing,et al.Studies on angiogenesis and endothelial cell apoptosis in radiation-combined wound healing in Wistar rats[J].Chin J Radiol Med Prot,2002,22(1):20-21 |
| 大鼠放射复合伤伤口中血管再生及内皮细胞凋亡研究 |
| Studies on angiogenesis and endothelial cell apoptosis in radiation-combined wound healing in Wistar rats |
| 投稿时间：2001-02-20 |
| DOI： |
| 中文关键词: 伤口愈合 辐射 血管再生凋亡 |
| 英文关键词:Wound healing Radiation Angiogenesis Apoptosis |
| 基金项目:全军“九五”重点招标课题基金资助项目(96Z008) |
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| 中文摘要: |
| 目的 探讨放射复合伤伤口中血管再生、内皮细胞凋亡及意义。方法 Wistar大鼠皮肤伤口以25Gy60Coγ射线局部照射, 于伤后2、5、10、15、21和28d活杀取材, 采用原位末端标记、图像分析等技术研究血管再生、内皮细胞改变。结果 单创组血管再生始于伤后2d,于10d其数量达高峰, 15d后渐减少, 此时凋亡的内皮细胞增多, 坏死偶见；伤照组血管再生始于伤后5d,可见内皮细胞凋亡, 15d其数量达高峰, 21d坏死和凋亡的内皮细胞均增多。结论 辐射使血管再生延迟, 内皮细胞提早凋亡, 高峰滞后；辐射使血管再生障碍是辐射延迟伤口愈合的原因之一。 |
| 英文摘要: |
| Objective To study the changes and significance of angiogenesis and endothelial cell apoptosis in radiation-combined wound healing. Methods Wistar rats were wounded and then local irradiation was performed immediately with a dose of 25 Gy 60 Co γ-rays in the irradiation wound group.Tissue specimens were obtained at 2,5,10,15,21 and 28 days after injury and angiogenesis and apoptosis of endothelial cells were investigated by means of LM,EM and in situ terminal labeling. Results In the nonirradiation wound group,angiogenesis began from day 2 and the capillary mumber reached its peak on day 10 and decreased on day 15 after injury when the endothelial cells occurred apoptosis and scarcely necrosis.In the irradiation wound group angiogenesis began on day 5 when endothelial cells occurred apoptosis.The capillary number reached its peak on day 15 and decreased on day 21 after injury when endothelial cells occurred both apoptosis and necrosis. Conclusions Radiation may delay the angiogenesis in wound healing,induce apoptosis of endothelial cells earlier and delay the peak of the capillary number.That radiation may delay the angiogenesis is one of reasons in delay of wound healing by radiation. |
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