| 吴刚,顾红光,韩本立,等.γ射线诱导癌细胞凋亡致敏树突状细胞后的免疫应答[J].中华放射医学与防护杂志,2002,22(1):13-16.WU Gang,GU Hongguang,HAN Benli,et al.Immune responses of dendritic cells after acquiring antigen from apoptotic hepatochlangioma cells caused by γ-ray[J].Chin J Radiol Med Prot,2002,22(1):13-16 |
| γ射线诱导癌细胞凋亡致敏树突状细胞后的免疫应答 |
| Immune responses of dendritic cells after acquiring antigen from apoptotic hepatochlangioma cells caused by γ-ray |
| 投稿时间：2001-03-24 |
| DOI： |
| 中文关键词: γ射线 树突状细胞 粒细胞-巨噬细胞集落刺激因子 白细胞介素4 凋亡 细胞毒T淋巴细胞 |
| 英文关键词:ray Dendritic cells(DCs) GM-CSF IL-4 Apoptosis CTLs |
| 基金项目:国家杰出青年科学基金资助项目(39825111) |
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| 中文摘要: |
| 目的 观察树突状细胞(DCs)从γ射线诱导的凋亡胆管癌细胞获取抗原后, 抗肿瘤免疫应答及对胆管癌细胞的特异性免疫杀伤效果。方法 用粒巨噬细胞集落刺激因子(GM-CSF)加白介素4(IL-4)从人外周血分化、诱导DCs,γ射线在体外诱导培养的人胆管癌细胞凋亡, 将DCs、T淋巴细胞和凋亡胆管癌细胞共培养, 同时设计不同类型肿瘤细胞(坏死胆管癌细胞及培养胆管癌细胞)作对照, 7d后, 分离、富集DCs、T淋巴细胞进行免疫应答及肿瘤细胞杀伤试验。结果 与凋亡胆管癌细胞共培养之DCs可以有效提呈胆管癌细胞抗原, 有强烈的免疫应答, 刺激的细胞毒T淋巴细胞(CTLs)特异性地杀伤胆管癌细胞。结论 γ射线诱导癌细胞凋亡可以致敏rhGMCSF加rhIL-4从人外周血单核细胞诱导、扩增出的DCs并产生显着的杀伤胆管癌细胞的免疫反应, 可望成为特异性免疫治疗肿瘤的一条新途径。 |
| 英文摘要: |
| Objective To investigate the induction of cytotoxic T lymphocytes (CTLs) in antitumor responsiveness and therapeutic effects after dendritic cells (DCs) acquired antigen from apoptotic hepatochlangionma cells. Methods DCs from blood mononuclear cells that maintain the characteristics of immaturity-antigen-capturing and-processing capacity were established in vitro by using granulocyte/macrophage colony-stimulating factor (GM-CSF) and interleukin-4.Then,apoptosis in hepatochlangioma cells was induced with γ-radiation.The experimental groups included (1) coculture of DCs,and apoptotic cancer cells and T cells(2)coculture of DCs necrotic cancer cells and T cells(3)coculture of DCs-cultured cancer cell and T cells.These cells were cocutured for 7 days.DCs and T cell were enriched separately.Finally,antitumor response test was carried out. Results These cells had typical dendritic morphology,expressed high levels of CD1a,B7 and acquired antigen from apoptotic cells caused by γ-rays and induced an increased T cell-stimulatory capacity in MLR. Conclusions DCs obtained from blood mononuclear cells using GM-CSF and IL-4 and DCs can efficiently present antigen drived from apoptotic cells caused by γ-rays and induce T cells increasing obviously.It can probably become an effective approach of DC transduction with antigen. |
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