| 卢红梅,强亦忠,施勤,顾惠心,王崇道,张学光.60Coγ射线照射诱导人卵巢癌细胞凋亡及细胞周期的改变[J].中华放射医学与防护杂志,2001,21(6):435-437 |
| 60Coγ射线照射诱导人卵巢癌细胞凋亡及细胞周期的改变 |
| Apoptosis and cell cycle alternations in human ovarian cancer cell lines induced by 60Coγ-irradiation |
| 投稿时间：2001-02-13 |
| DOI： |
| 中文关键词: 卵巢肿瘤 辐射敏感性 细胞周期 细胞凋亡 |
| 英文关键词:Ovarial tumor Radiosensitivity Cell cycle Apoptosis |
| 基金项目:核工业基金资助项目(Y5573162) |
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| 中文摘要: |
| 目的 探讨60Coγ射线照射对人卵巢癌细胞株A2780及其顺铂耐药株A2780-CP体外生长、凋亡及细胞周期的影响。方法 采用四甲基偶唑蓝(MTT)比色法分析不同辐照剂量(1～10Gy)对A2780及A2780-CP两种细胞株体外生长的影响,用流式细胞术(FCM)比较两者辐照后凋亡及细胞周期的变化。结果 (1)1～10Gy60Coγ射线照射引起A2780细胞株明显的生长抑制和凋亡,而A2780-CP细胞则表现出明显的辐照抗性。(2)6Gy60Coγ射线照射后6～48hA2780细胞呈现G1期细胞阻滞和S期细胞比例下降,A2780-CP细胞则呈G1期细胞比例减少和S期细胞比例增加。结论卵巢癌耐药细胞具有辐射抗性和特征性的细胞周期变化,流式细胞术测定辐照诱导肿瘤细胞凋亡及细胞周期的变化可预测恶性肿瘤细胞的辐射敏感性。 |
| 英文摘要: |
| Objective To Compare the proliferaitve capacity,apoptosis and cell cycle alterations of human ovarian cancer cell line A2780 and its cisplatin-resistant subline A2780-CP in vitro after 60Coγ irradiation. Methods The effects of in vitro proliferative capacity of both A2780 and A2780 CP cell lines irradiated with 1～ 10 Gy gamma-rays were evaluated with microculture tetrazolium(MTT) colourmetry.The apoptosis and cell cycle of the above-mentioned cell lines were analyzed with flow cytometry. Results (1)60 Coγ-irradiation inhibited the growth of A2780 cells significantly and induced their apoptosis in a dose-dependent manner,while A2780-CP cells exhibited higher radioresistance.(2) The G1 phase cells arrested progressively and the ratio of Sphase cell decreased in A2780 cells,while the ratio of G1 phase cells and of Sphase cells increased in A2780-CP cells after exposure to 6 Gy 60Coγ-irradiation in a time-dependent manner. Conclusion The cisplatin-resistant cell line of ovarian cancer is significantly radioresistant and exhibits characteristic alterations in cells cycle after irradiation.Radiation induced apoptosis and cell cycle alterations revealed by flow cytometry can foretell the radiosensitivity of human malignant tumour cells in vitro. |
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