| 朱剑昆,张学光,许志祥,徐颖,赵仁亮,於葛华,强亦忠.重组人Flt3配体联合其它细胞因子对免疫细胞的体外抗辐射作用研究[J].中华放射医学与防护杂志,2001,21(6):424-427 |
| 重组人Flt3配体联合其它细胞因子对免疫细胞的体外抗辐射作用研究 |
| Radioprotective effects of recombinant human Flt3 ligand and other cytokines on immunocytes in vitro |
| 投稿时间：2000-11-21 |
| DOI： |
| 中文关键词: 细胞因子 Flt3配体 免疫细胞 辐照 凋亡 |
| 英文关键词:Cytokines Flt3 ligand Immune cells Irradiation Apoptosis |
| 基金项目:国防科工委国防预研基金资助项目(Y5573162);国际原子能机构(IAEA)基金资助项目(CRP/9/025) |
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| 中文摘要: |
| 目的 研究重组人Flt3配体(rhFL)及其他细胞因子在体外对免疫细胞的抗辐射作用及其可能机理。方法 用流式细胞仪分析照射前后外周血单个核细胞(PBMC)表型及rhFL对受照射PBMC表型的影响。[3H]TdR测定细胞的增殖能力。PBMC来源贴壁细胞经细胞因子诱导树突状细胞(DC),并观察辐射对诱导DC的影响及rhFL对DC的抗辐射效应;用PI-AnnexinV、DNA Ladder分析细胞凋亡。结果 PBMC在体外经15Gy照射后,CD8+、CD56+细胞明显减少,照射前细胞培养液中加入100ngmlrhFL,可促进CD56+细胞的恢复;照射前应用rhFL及照射后联合应用IL-2等细胞因子,可促进受照射CD8+、CD56+细胞的恢复;rhFL可协同GMCSF、IL-4、TNFα产生DC,并使DC细胞的数量较常规方法诱导产生的数量增加6倍,而且rhFL对DC分化诱导的辐射抑制作用具有明显的抗性;rhFL可抑制照射所致的细胞凋亡。结论 rhFL单独或与其他细胞因子联合应用,对人外周血免疫细胞具有明显的抗辐射效应,这对辐射损伤机体免疫功能的调节和恢复具有重要的使用价值 |
| 英文摘要: |
| Objective To investigate the immunoprotective potential of recombinant human Flt3 ligand (rhFL) and other cytokines on irradiated peripheral blood mononuclear cells (PBMC) in vitro . Methods The phenotypes of PBMCwere analysed by flow cytometric analysis.The proliferative-promoting effect of rhFL on irradiated PBMC was measured by 3H-thymidine incorporation assay.Dendritic cells (DC) were induced from irradiated PBMC by a cytokine cocktail with or without rhFL.Apoptotic rate of irradiated PBMC was measured with PI-Annexin Vand DNAladder. Results CD8+ and CD56+ cells in PBMC decreased significantly at 24 h and 48 h post-irradiation.If the PBMC were cultured with rhFL before irradiation and with IL-2 after irradiation CD8+,CD56+ cells increased and approached to the non-irradiated level.The number of DC induced from irradiated PBMC increased by the addition of rhFL.rhFL stimulated the proliferation of the PBMC,but its effect was weaker than that of PHA.Irradiation caused a significant increase of apoptosis in PBMC.Addition of rhFL to the culture reduced apoptosis of PBMC significantly. Conclusion rhFLprotects the immunocytes in 15 Gy γ-irradiation treated PBMC from apoposis,so it shows promising irradiation-protective effects on immune system. |
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