| 邓宇斌,李树浓,周平坤,Teyssier Magali.辐射诱导人细胞系CEM、外周血单个核细胞DNA修复基因hHR21sp的表达研究[J].中华放射医学与防护杂志,2001,21(6):413-416 |
| 辐射诱导人细胞系CEM、外周血单个核细胞DNA修复基因hHR21sp的表达研究 |
| Studies on hHR21sp expression induced by irradiation in the peripheral blood cells and human cell line (CEM) |
| 投稿时间：2001-04-17 |
| DOI： |
| 中文关键词: 电离辐射 基因表达 hHR21sp基因 |
| 英文关键词:Ionizing radiation(IR) Gene expression Gene hHR21 |
| 基金项目:CMB(美国中华医学基金会)基金(96630);2000年教育部回国人员启动基金资助项目(2000年度) |
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| 中文摘要: |
| 目的 研究辐射对人T淋巴细胞白血病细胞系(CEM)、外周血单个核细胞的hHR21sp基因转录表达水平的影响及意义。方法 分别对人T淋巴细胞白血病细胞系CEM和正常人外周血单核细胞在UV或γ辐射后不同时间提取细胞总RNA,通过RT-PCR与hHR21sp基因特异引物杂交,以β-actin为内参照放射影像密度扫描检测人T淋巴细胞白血病细胞系CEM、单核细胞DNA修复基因表达。结果 在UV-辐射、γ-辐射后早期(3～6h),人T淋巴细胞白血病细胞系CEM和淋巴细胞hHR21sp基因的表达水平明显增加,照射后6hhHR21sp基因的表达水平增加最多且UV辐射更明显,在晚期(9h)降低。比较人细胞系CEM和淋巴细胞两者hHR21sp基因的表达水平,γ辐射(3Gy)后淋巴细胞对hHR21sp基因表达高于人细胞系CEM,且表达增加时间较长,达9h;而人细胞系CEM受到γ辐射后早期表达增加,在6～9h后表达降低。结论 人T淋巴细胞白血病细胞系(CEM)和人淋巴细胞的DNA修复基因hHR21sp基因在一定剂量辐射(UV、γ辐射)范围内其表达水平随辐射剂量增加而诱导表达水平增高,且对UV辐射更敏感;提示hHR21sp基因在人细胞系CEM细胞和人单核细胞在照射损伤后表达增加,可能是促进单核细胞损伤修复的原因之一。 |
| 英文摘要: |
| Objective To study the effects of UV and gamma-ray irradiation on hHR21sp transcription in a human T lymphocytic leukemia cell line,CEM. Methods Total RNA was extracted from normal human peripheral blood mononuclear cells (PB-MNC) and CEM cells after exposure to UV and gamma-ray irradiation.The level of hHR21 transcripts was deteced using RT-PCR with southern blot hybridization and phosphorlmage autoradiogram analysis. Results hH21sp mRNAincreased significantly at 3 h to 9 h after UVirradiation in PB-MNC and CEM cells,and the induction doses were 40 to 80 J/Mand 80-120 J/Mfor PB-MNC and CEM cells,respectively.In PB-MNC,hHR21sp mRNA increased at 6 to 9 h after 1 to 5 Gy of gamma-ray irradiation.In contrast,hHR21sp mRNA was induced only at 3 h after irradiation in CEM cells,and its peak appeared at 9 h after irradiation with 3 Gy. Conclusion The results revealed that hHR21sp gene transcription could be induced by UVas well as gamma-ray irradiation.The levles of hHR21sp mRNA induced by gamma-ray irradiation was obviously different between PB-MNC and CEM cells. |
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