中华放射医学与防护杂志

刘佳梅,陈东,郑永晨,等.低剂量电离辐射对小鼠派伊氏板细胞Bcl-2转录水平的影响[J].中华放射医学与防护杂志,2001,21(6):410-412.LIU Jiamei,CHEN Dong,ZHENG Yongchen,et al.Effect of low dose ionizing radiation on Bcl-2 transcription level of Peyer's patches in mouse[J].Chin J Radiol Med Prot,2001,21(6):410-412

低剂量电离辐射对小鼠派伊氏板细胞Bcl-2转录水平的影响

Effect of low dose ionizing radiation on Bcl-2 transcription level of Peyer's patches in mouse

投稿时间：2001-10-28

DOI：

中文关键词: X射线派伊氏板 Bcl-2

英文关键词:X-rays Peyer's patches Bcl-2

基金项目:国家教委回国人员科研启动资金;国家自然科学基金资助项目(39570188)

作者	单位
刘佳梅	吉林大学白求恩医学部组胚教研室, 长春 130021
陈东	吉林大学白求恩医学部组胚教研室, 长春 130021
郑永晨	吉林大学第一临床学院中心实验室
刘树铮	吉林大学卫生部放射生物学重点实验室

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中文摘要:

目的研究不同剂量X射线全身照射对小鼠派伊氏板细胞凋亡的影响及作用机理。方法采用反转录PCR法检测了派伊氏板细胞Bcl-2转录水平的变化,并用琼脂糖凝胶电泳、流式细胞术检测了不同剂量X射线全身照射后小鼠派伊氏板细胞DNA及凋亡小体的改变。结果 2GyX射线全身照射使派伊氏板细胞凋亡增多,Bcl-2转录水平表达下降;而75mGy X射线全身照射使派伊氏板细胞凋亡减少,同时Bcl-2转录水平表达升高。结论 Bcl-2参与了辐射诱导小鼠派伊氏板细胞凋亡的基本调控。

英文摘要:

Objective To study the effect of whole body irradiation (WBI) with different doses of X-rays on apoptosis in cells of mouse Peyers patches and its molecular mechanism. Methods RT-PCRwas used to detect the changes of Bcl-2 transcription level.Agarose electrophoresis and flow cytometry were used to detect the changes of DNAand apoptotic bodies in Peyers patches after WBIwith different doses of X-rays. Results The apoptotic was increased and Bcl-2 transcription level was decreased in Peyers patches after 2 Gy X-rays.The apoptotic rate was decreased and Bcl-2 transcription level was increased in Peyers patches after 75 mGy X-rays. Conclusion Bcl-2 participates in the regulation of radiation-induced apoptosis in Peyer's patches.

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