| 夏英,杨梅英,吕慧敏,冯治孝,叶常青,王作元.室内氡暴露居民肺癌p53和k-ras基因突变[J].中华放射医学与防护杂志,2001,21(6):404-405 |
| 室内氡暴露居民肺癌p53和k-ras基因突变 |
| p53 and k-ras mutations in lung cancer associated with residential radon exposure |
| 投稿时间：2001-04-17 |
| DOI： |
| 中文关键词: 氡 肺癌 p53 PCR-SSCP 序列分析 |
| 英文关键词:Radon Lung cancer p53 PCR-SSCP Sequence analysis |
| 基金项目:国家自然科学基金资助项目(39970235) |
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| 中文摘要: |
| 目的 检测室内高氡暴露地区居民肺癌p53和k-ras基因突变。方法 采用PCR扩增、PCR-SSCP电泳和序列分析方法。结果 9例标本中有5例p53PCR-SSCP电泳呈阳性结果,其中3例经克隆测序发现有碱基置换。结论 室内氡浓度在200～350Bq·m-3·a-1范围内的7例肺癌病例中有5例发生p53基因突变,p53基因突变还可能与肺癌患者的年龄及吸烟史有关。 |
| 英文摘要: |
| Objective The aim of this study was to analyze mutation of p53 and k-ras genes in lung cancer related to residential radon exposure. Methods PCR amplification,PCR-SSCP electrophoresis,and sequence analysis were carried out. Results Five of 9 lung cancer cases were found to be positive with p53 PCR-SSCP electrophoresis techniques,and in 3 among the 5 base substitution was observed. Conclusion Five of the seven cases of lung cancer 200-350 Bq·m-3 ·a-1 radon level had p53 gene mutation,which might be related to age and smoking status of the patients. |
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