中华放射医学与防护杂志

夏英,杨梅英,吕慧敏,等.室内氡暴露居民肺癌p53和k-ras基因突变[J].中华放射医学与防护杂志,2001,21(6):404-405.XIA Ying,YANG Meiying,Lü Huimin,et al.p53 and k-ras mutations in lung cancer associated with residential radon exposure[J].Chin J Radiol Med Prot,2001,21(6):404-405

室内氡暴露居民肺癌p53和k-ras基因突变

p53 and k-ras mutations in lung cancer associated with residential radon exposure

投稿时间：2001-04-17

DOI：

中文关键词: 氡肺癌 p53 PCR-SSCP 序列分析

英文关键词:Radon Lung cancer p53 PCR-SSCP Sequence analysis

基金项目:国家自然科学基金资助项目(39970235)

作者	单位
夏英	北京卫生部工业卫生实验所 100088
杨梅英	北京放射医学研究所
吕慧敏	北京卫生部工业卫生实验所 100088
冯治孝	甘肃庆阳地区医院
叶常青	北京放射医学研究所
王作元	北京卫生部工业卫生实验所 100088

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中文摘要:

目的检测室内高氡暴露地区居民肺癌p53和k-ras基因突变。方法采用PCR扩增、PCR-SSCP电泳和序列分析方法。结果 9例标本中有5例p53PCR-SSCP电泳呈阳性结果,其中3例经克隆测序发现有碱基置换。结论室内氡浓度在200～350Bq·m^-3·a^-1范围内的7例肺癌病例中有5例发生p53基因突变,p53基因突变还可能与肺癌患者的年龄及吸烟史有关。

英文摘要:

Objective The aim of this study was to analyze mutation of p53 and k-ras genes in lung cancer related to residential radon exposure. Methods PCR amplification,PCR-SSCP electrophoresis,and sequence analysis were carried out. Results Five of 9 lung cancer cases were found to be positive with p53 PCR-SSCP electrophoresis techniques,and in 3 among the 5 base substitution was observed. Conclusion Five of the seven cases of lung cancer 200-350 Bq·m^-3 ·a^-1 radon level had p53 gene mutation,which might be related to age and smoking status of the patients.

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