| 谷庆阳,王德文,高亚兵,周洁,秦全红,彭瑞云,夏国伟,崔玉芳,杨红.MMP1在放射复合伤口愈合过程中的表达及对伤口愈合和组织改建的影响[J].中华放射医学与防护杂志,2001,21(5):342-345 |
| MMP1在放射复合伤口愈合过程中的表达及对伤口愈合和组织改建的影响 |
| Expression of MMP1 in healing of radiation-combined wounds and its effects on healing process and tissue remodeling |
| 投稿时间：2000-10-15 |
| DOI： |
| 中文关键词: 放射 伤口愈合 基质金属蛋白酶-1(MMP1) |
| 英文关键词:Irradiation-Wound healing Matix metalloproteinases1(MMP1) |
| 基金项目:全军"九五"医药卫生科研基金课题重点项目(96Z008) |
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| 中文摘要: |
| 目的 观察基质金属蛋白酶-1(MMP1)在放射复合伤口愈合过程中的表达及对伤口愈合和组织改建的影响.方法 利用本实验室已建立的放射复合伤口动物模型,采用光镜、电镜、免疫组化及原位杂交等方法,动态观察伤口愈合过程中MMP1mRNA转录及蛋白表达的变化.结果 25Gy(γ射线)局部照射对伤口愈合有明显的损害作用,照射组伤口较对照组伤口延迟6d愈合.①在伤口愈合的炎症期和肉芽组织生长期,照射组新生表皮细胞中MMP1表达强度与对照组相近,在愈合后期随着照射组伤口愈合的延迟其表达相对增高.②MMP1在照射组肉芽组织成纤维细胞、血管内皮细胞、巨噬细胞中表达明显降低,在愈合后期因辐射所致伤口愈合的延迟同样表现为表达时相拖后.结论 MMP1在放射复合伤口肉芽组织中表达明显降低直接影响细胞迁移、血管形成和基质改建等病理过程,是伤口愈合延迟的重要机理之一;愈合早、中期MMP1在表皮细胞中的表达有利于重上皮化,后期在放射伤口中的高表达可能影响表皮基底膜和肉芽组织的重建. |
| 英文摘要: |
| Objective To study the expression of MMP1 in healing of simple and radiation-combined wounds and its effects on the healing process and tissue remodeling. Methods Arat model of radiation-combined wound healing was used.Routine light microscopy,electron microscopy,immunohistochemistry,and %in situ % hybridization were performed to detect MMP1 expression during the healing process. Results The wound healing process was impaired and delayed in radiation-combined wound (RCW).In rats receiving 25 Gy γ-rays locally the RCW(s) healed 6 days later than non-irradiated ones do.The following changes in MMP1 expression were found:① In the early inflammatory phase and in the period of granulation tissue formation,MMP1 expression was only slightly,if any,affected in the newly-formed epidermis of RCWcompared with controls.Later,the epidermal expression of MMP1 in RCWwas comparatively increased along with the delay of the healing process.② MMP1 expression in RCWwas markedly decreased in fibroblasts,endotheliocytes and macrophages compared to controls.The expression phase was prolonged due to the delay of the healing process. Conclusion We conclude that the reduced expression of MMP1 in granulation tissue retards such important processes as cell migration,angiogenesis and tissue remodeling,thus slowing down the healing process.The expression of MMP1 in the proliferating keratinocytes may help the process of reepithelialization;but in the late healing period,overexpression of MMP1 in the epidermis may hinder the establishment of basal membrane and the formation of granulation tissue,and also affect the matrix remodeling process. |
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