| 刘佳梅,陈东,陈爱军,刘树铮.X射线全身照射诱导小鼠派伊氏板细胞凋亡及其机理的研究[J].中华放射医学与防护杂志,2001,21(5):339-341 |
| X射线全身照射诱导小鼠派伊氏板细胞凋亡及其机理的研究 |
| Studies on induction of apoptosis in mouse Peyer's patches by whole body X-irradiation and its mechanisms |
| 投稿时间：2000-11-10 |
| DOI： |
| 中文关键词: X射线 派伊氏板 细胞凋亡 Bcl-xL Fas-L |
| 英文关键词:X-rays Peyer's patches Apoptosis Bcl-xL Fas-L |
| 基金项目:国家教委回国人员科研启动资金;国家自然科学基金资助项目(39570188) |
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| 中文摘要: |
| 目的 研究不同剂量X射线全身照射后小鼠派伊氏板细胞凋亡相关基因蛋白表达的变化规律,初步阐明凋亡调控的部分分子机理.方法 应用光、电镜技术观察小鼠派伊氏板细胞凋亡的形态改变,并用流式细胞术检测小鼠派伊氏板细胞Bcl-xL及FasL蛋白表达的变化.结果 2GyX射线全身照射后派伊氏板细胞凋亡增加,且Bcl-xL蛋白表达下降,FasL蛋白表达升高;75mGyX射线全身照射后,派伊氏板细胞凋亡减少,且Bcl-xL蛋白表达升高,FasL蛋白表达下降.结论 凋亡相关基因Bcl-xL、FasL在电离辐射诱导的派伊氏板细胞凋亡方面起重要的调控作用. |
| 英文摘要: |
| Objective To study the effect of apoptosis-related genes in mouse Peyer's patches and its molecular mechanism after whole body irradiation(WBI) with different doses of X-rays. Methods Light microscopy and transmission electron microscopy were used to observe the morphology and structure of Peyer's patches and flow cytometry (FCM) was used to detect the changes of Bcl-xL and Fas-Lproteins. Results The apoptosis in Peyer's patches was increased after 2 Gy X-rays and decreased after 75 mGy X-rays.The expression of Bcl-xL protein was decreased and the expression of Fas-Lprotein was increased in Peyer's patches after WBIwith 2 Gy X-rays,whereas the former increased and the latter decreased after WBIwith 75 mGy. Conclusion Apoptosis-related genes such as Bcl-xL,Fas-Lmight play an important role in regulation of radiation-induced apoptosis in Peyer's patches. |
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