| 任晓庆,黄定九,黄钢,等.辐射致大鼠平滑肌细胞HPRT基因第7/8外显子突变分析[J].中华放射医学与防护杂志,2001,21(5):328-331.REN Xiaoqing,HUANG Dingjiu,HUANG Gang,et al.Analysis of mutation induced by radiation in HPRT gene exon 7/8 of rat smooth muscles cells[J].Chin J Radiol Med Prot,2001,21(5):328-331 |
| 辐射致大鼠平滑肌细胞HPRT基因第7/8外显子突变分析 |
| Analysis of mutation induced by radiation in HPRT gene exon 7/8 of rat smooth muscles cells |
| 投稿时间：2000-12-10 |
| DOI： |
| 中文关键词: 基因突变 188Re HPRT PCR-SSCP |
| 英文关键词:Ionizing Gene mutation 188Re HPRT PCR-SSCP |
| 基金项目:国家自然科学基金资助项目(39970221) |
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| 中文摘要: |
| 目的 探讨辐射剂量与平滑肌细胞HPRT基因变异的关系,提供放射预防血管再狭窄的基础实验依据.方法 不同剂量的放射性核素188Re内辐射平滑肌细胞后,应用6TG筛选分离HPRT变异细胞,采用PCRSSCP技术进行HPRT基因78外显子的变异分析.结果 辐射后平滑肌细胞HPRT基因突变率为(55～13)×10-6;91株HPRT突变细胞克隆中,143%呈现78外显子缺失,165%呈现点突变,78外显子总变异率为308%;辐射剂量与HPRT基因突变率、第78外显子的总变异率及外显子缺失率呈正相关.结论 辐射所致DNA分子的损伤,包括基因片段的缺失、断裂及点突变与辐射剂量呈正相关. |
| 英文摘要: |
| Objective To investigate the relationship between radiation dose and HPRT gene locus mutation in rat smooth muscle cells,and provide a molecular basis for prevention of blood vessel restenosis after PTCA. Methods The smooth muscle cells cultured in vitro were irradiated by radionuclide 188Re with different doses.HPRT gene mutation colonies were selected and isolated by 6-thioguanine.Analysis of mutation in exon 7/8 of HPRT gene were accomplished by polymerase chain reaction and single-strand conformation polymorphism. Results The HPRTgene mutation frequency of rat smooth muscle cells that were irradiated by radionuclide 188Re ranged from 55×10-6 to 13×10-6. of 91 HPRT gene mutation colonies,13 contained exon 7/8 deletion and 15 had point mutation.The exon 7/8 mutation frequency was 308%.There was significant relationship between radiation dose and mutation frequency of HPRTgene and exon 7/8. Conclusions The DNA damage and gene mutation induced by radiation was the basis of proliferation inhibition and apoptosis of smooth muscle cells. |
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