| 王菊芳,周光明,李文建,李强,党秉荣,何静,卫增泉,高清祥,陈宏波.不同LET碳离子对哺乳动物细胞的生物学效应[J].中华放射医学与防护杂志,2001,21(5):325-327 |
| 不同LET碳离子对哺乳动物细胞的生物学效应 |
| Biological effects on mammalian cells of carbon ions with different LET |
| 投稿时间：2000-12-10 |
| DOI： |
| 中文关键词: 传能线密度 碳离子 哺乳动物细胞 存活率 微核率 染色体畸变 |
| 英文关键词:Linear energy transfer Carbon ions Mammalian cell Surviving fraction Micronucleus frequency Chromosomal aberration |
| 基金项目:中科院西部之光资助项目(XB980604) |
|
| 摘要点击次数: 2617 |
| 全文下载次数: 1921 |
| 中文摘要: |
| 目的 研究不同传能线密度(LET)的重离子对体外培养细胞存活率、微核率和染色体畸变的效应.方法 用集落形成法研究细胞的存活情况,观察统计细胞微核率和染色体结构的变化.结果 ①细胞存活:不同LET碳离子引起细胞失活效应由大到小的顺序依次为125,200,700keV/μm.②细胞微核:相同剂量下,200keV/μm碳离子所致微核率高于700keV/μm时的结果.③染色体畸变:可看到多种染色体结构变异.结论 在本实验中,LET为125keV/μm的碳离子引起的失活效应最大,200keV/μm碳离子引起的微核率高于700keV/μm碳离子引起的微核率. |
| 英文摘要: |
| Objective The effects of cells carbonions with different linear energy transfer(LET) on survival fraction,micronucleus frequency and chromosomal aberrations of cultured cells were investigated. Methods The survival fraction was studied by colong-formation assay and the mironucleus frequency and structural aberration of chromosomes were observed and counted. Results ①Survival fraction:The LET order of carbon ions from high to low inducing celluar inactivation was 125,200 and 700 keV/μm.②Micronucleus frequency:Under the same dose,the micronucleus frequency induced by 200 keV/μm carbon ions was higher than that by 700 keV/μm ones.③Chromosomal aberration:Alot of structural aberrations could be observed. Conclusion In this study,the maximum celluar inactivation was obtained when LETof carbon ions was 125 keV/μm,the micronucleus frequency caused by carbon ions of 200 keV/μm was higher than that caused by 700 keV/μm. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|