中华放射医学与防护杂志

夏劲松,吴华,赵明,鲜于志群.^99mTc-MIBI监测肿瘤照射后生物学效应的实验研究[J].中华放射医学与防护杂志,2001,21(5):317-320

^99mTc-MIBI监测肿瘤照射后生物学效应的实验研究

Monitoring biological responses of tumor cells after irradiation with ^99mTc-MIBI

投稿时间：2000-11-10

DOI：

英文关键词:_99mTc-MIBI Tumor cell Irradiation Biology effect

基金项目:

作者	单位
夏劲松	华中科技大学同济医学院附属同济医院核医学科, 武汉 430030
吴华	华中科技大学同济医学院附属同济医院核医学科, 武汉 430030
赵明	华中科技大学同济医学院附属同济医院核医学科, 武汉 430030
鲜于志群	华中科技大学同济医学院附属同济医院核医学科, 武汉 430030

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中文摘要:

目的研究照射后肿瘤细胞摄取MIBI能力的变化及其与肿瘤细胞凋亡、坏死和增殖状态的关系.方法将离体肿瘤细胞和实验动物按照射剂量0、5、10、20Gy与照射后24、48、72h进行分组比较.离体肿瘤细胞分别测定单位质量肿瘤细胞摄取^99mTc-MIBI的放射性计数值(计数·min^-1·mg^-1cell protein),凋亡指数(Apoptosis Index,AI),集落形成率(Plating efficiency,PE).实验动物计算肿瘤组织微分摄取率(differential uptake ratio,DUR)和AI值.结果不同剂量照射后,肿瘤细胞的^99mTc-MIBI摄取值与AI值呈负相关(r=-0.93),与PE值呈正相关(r=0.84).24hDUR值与AI负相关(r=-0.793).结论不同剂量照射后,肿瘤细胞活力变化可能用^99mTc-MIBI作为示踪剂得到反映.

英文摘要:

Objective To explore the possibility of using _99mTc-MIBI to monitor biological responses of tumor cells after irradiation. Methods To observe the relation between the irradiation doses and the uptake levels of _99mTc-MIBI by tumor cells irradiated with a single dose of 5 Gy,10 Gy and 20 Gy,respectively.The uptake of _99mTc-MIBI in each dose group was determined before and at 24,48,72 hours after irradiation.Apoptosis index (AI) and plating efficiency (PE) of tumor cells were determined simultaneously.The relation between the uptake levels of _99mTc-MIBI in vivo (expressed as differential uptake ratio,DUR) and AIwas also determined. Results There was a negative correlation between uptake levels of _99mTc-MIBI and AI ( r=-093). Apositive correlation was shown between the uptake levels and PE ( r= 084).At 24 h after irradiation,DUR was inversely correlated with AI( r=0793). Conclusion It is suggested that 99m Tc-MIBI might be used as a tracer to monitor the change of viability state of tumor cells after irradiation with different doses.

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