| 吕喆,刘淑春,孙祖玥,等.低剂量电离辐射诱导小鼠胸腺细胞凋亡及细胞周期进程适应性反应的时间效应[J].中华放射医学与防护杂志,2001,21(4):276-278.LU Zhe,LIU Shuchun,SUN Zuyue,et al.Time-effect of adaptive response of thymocyte apoptosis and cell cycle progression induced by low dose ionizing radiation in mice[J].Chin J Radiol Med Prot,2001,21(4):276-278 |
| 低剂量电离辐射诱导小鼠胸腺细胞凋亡及细胞周期进程适应性反应的时间效应 |
| Time-effect of adaptive response of thymocyte apoptosis and cell cycle progression induced by low dose ionizing radiation in mice |
| 投稿时间：2000-09-12 |
| DOI： |
| 中文关键词: 电离辐射 胸腺细胞 凋亡 细胞周期 适应性反应 |
| 英文关键词:Ionizing irradiation Thymocytes Apoptosis Cell cycle progression Adaptive response |
| 基金项目:国家自然科学基金资助项目(39170275) |
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| 中文摘要: |
| 目的 观察低剂量辐射诱导胸腺细胞凋亡及细胞周期进程适应性反应的基本规律.方法 实验用X射线照射昆明系雄性小鼠,其诱导剂量 (D1)及其后攻击剂量 (D2)分别是 75mGy和1.5Gy.D1 和D2 间隔时间分别是 3、6、12、2 4和 6 0h.通过流式细胞仪检测胸腺细胞凋亡和细胞周期进程的变化.结果 当D1 和D2 间隔 3、6和 12h,D1 +D2 组胸腺细胞凋亡百分数明显低于D2 组(P <0.0 5),G0 G1 和G2 +M期细胞百分数也不同程度地低于D2 组,而S期细胞百分数却明显高于D2 组 (P <0.0 5或P <0.0 1).结论 上述结果指出,D1 在 75mGy,吸收剂量率为 12.5mGy min;D2在 1.5Gy,吸收剂量率为 0.2 87Gy min;D1 和D2 间隔 3～12h,可在全身照射条件下诱导小鼠胸腺细胞凋亡和细胞周期进程的适应性反应. |
| 英文摘要: |
| Objective In the present study we observed the general pattern of the adaptive response of thymocyte apoptosis and cell cycle progression induced by low dose radiation. Methods Kunming male mice were irradiated with the inductive dose(D1,75 mGy)and the challenging dose(D2,1.5 Gy).The intervals between D1 and D2 were 3,6,12,24 and 60 hours separately.The changes of thymocyte apoptosis and cell cycle progression were measured with flow cytometry. Results When the intervals between D1 and D2 were 3,6 and 12 hours,the percentages of apoptotic cells in D1+D2 groups were significantly lower than those in D2 groups (P< 0.05),and the percentages of G0/G1 and G2+M phase cells decreased in varying degrees,while the percentages of S phase cells increased significantly(P<0.05 or P<0.01). Conclusion The results mentioned above indicate that when the mice were irradiated with 75 mGy(D1,12.5 mGy/min)3～12 hours before 1.5 Gy(D2,0.287 Gy/min)exposure,the adaptive response of thymocyte apoptosis and cell cycle progression could be induced under the condition of whole body irradiation. |
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