| 石梅,魏丽春,孙朝阳,等.放疗对人宫颈癌组织的增殖细胞核抗原(PCNA)和c-fos表达的影响[J].中华放射医学与防护杂志,2001,21(4):273-275.SHI Mei,WEI Lichun,SUN Chaoyang,et al.Influence of radiotherapy on expression of the proliferating cell nuclear antigen(PCNA) and c-fos in human cervical cancer[J].Chin J Radiol Med Prot,2001,21(4):273-275 |
| 放疗对人宫颈癌组织的增殖细胞核抗原(PCNA)和c-fos表达的影响 |
| Influence of radiotherapy on expression of the proliferating cell nuclear antigen(PCNA) and c-fos in human cervical cancer |
| 投稿时间：2000-12-05 |
| DOI： |
| 中文关键词: 组织增殖细胞核抗原 c-fos 宫颈癌 放射治疗 |
| 英文关键词:Proliferating cell nuclear antigen c-fos Human cervical cancer Radiotherapy |
| 基金项目:国家自然科学基金资助项目(30040012) |
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| 中文摘要: |
| 目的 探讨照射前、后人宫颈癌组织增殖细胞核抗原 (PCNA)和c-fos表达的变化.方法 在多聚甲醛固定的活检癌组织冰冻切片上,进行PCNA和Fos的免疫组织化学染色.结果 放疗前的宫颈癌组织内,多数癌细胞呈PCNA免疫染色阳性;放疗后,多数癌细胞无PCNA染色,但间质细胞的PCNA免疫染色明显升高,大量间质细胞则呈中等或强阳性染色.放射治疗前的宫颈癌组织内,多数癌细胞呈Fos免疫染色阳性;放疗后的宫颈癌组织Fos免疫染色显着降低,多数癌细胞无Fos染色,但放疗前、后间质细胞的Fos免疫反应无明显变化.结论 照射抑制宫颈癌细胞的PCNA和c-fos表达的同时,诱导间质细胞的PCNA表达.提示PCNA和c-fos的表达状态可作为放射治疗中评价宫颈癌细胞增殖和间质组织修复的分子指标之一. |
| 英文摘要: |
| Objective To investigate changes of proliferating cell nuclear antigen(PCNA)expression in human cervical cancer following irradiation. Methods Immunohistochemical staining for PCNA was performed in frozen sections of formalin-fixed cervical cancer biopsy tissues. Results The majority of the cancer cells showed PCNA-immunoreactivity before irradiation.Following irradiation(30-40 Gy/15-20 f)PCNA-immunopositive staining was hardly detectable in most of the cancer cells.The PCNA-immunoreactivity,however, increasd after radiotherapy and moderate or heavy immunopositive staining for PCNA was seen in irradiated mesenchymal tissue cells. On the other hand after irradiation Fos-immunoreactivity decreased remarkably and Fos-immunoposi-five staining was hardly detectable in most of cancer cells. No obvious change in Fos-immunoreactivity however was seen in mesenchymal connective tissue following irradiation. Conclusion Irradiation inhibits PCNA and c-fos expression in ceivicval cancer cells whereas it induces the expression of PCNA in mesenchymal tissue cells The present results suggest that expression of PCNA and c-fos may be regarded as a molecular marker for evaluat-ing the cancer cell proliferation and mesenchymal tissue repair during radiotherapy of human cervical cancer. |
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