| 古桂雄,朱寿彭,王六一,杨淑琴,朱玲俐.浓缩铀对新生大鼠脑NSE和IL1-β含量变化的研究[J].中华放射医学与防护杂志,2001,21(4):269-272 |
| 浓缩铀对新生大鼠脑NSE和IL1-β含量变化的研究 |
| Influence of intracerebral exposure to enriched uranium on neuron specific enolase and interleukin-1 β content in neonatal rats |
| 投稿时间：2000-10-29 |
| DOI： |
| 中文关键词: 浓缩铀 新生大鼠 脑损伤 神经元特异性烯醇化酶 白细胞介素-1β |
| 英文关键词:Enriched uranium Neonatal rat Brain damage Neuron specific enolase(NSE) Interleukin-1β(IL-1β) |
| 基金项目:国家自然科学基金资助项目(39470389) |
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| 中文摘要: |
| 目的 研究α辐射体浓缩铀对新生大鼠脑发育的影响.方法 新生大鼠侧脑室注射2 μl铀溶液后,多方位观察不同浓度的浓缩铀对早期体格生长、行为发育的影响,放射自显影示踪术观察脑内放射性核素行径定位,放射免疫技术检测神经元特异性烯醇化酶、白细胞介素-1β的含量变化.结果 新生大鼠脑注入浓缩铀后,放射性核素行径主要定位于细胞核中,在细胞浆和细胞间隙中亦有呈现.可导致新生大鼠生长延迟及神经行为异常.大鼠的小脑、皮质、海马、间脑中神经元特异性烯醇化酶 (NSE)、白细胞介素-1β(IL-1β)的含量随着浓缩铀235U脑内浓度的增加,NSE含量下降,IL-1β却显着上升,并有浓度反应依赖关系. 结论 α辐射体浓缩铀在新生大鼠中对发育脑损伤的作用特征具有神经细胞的敏感性、易脆性和代偿性. |
| 英文摘要: |
| Objective To examine biochemically the injurious effects of enriched uranium 235U on developing brain of neonatal rats. Methods Neonatal rats were irradiated with single injection of 2 μl enriched uranium into the left lateral ventricle of the brain at postnatal day 1(<24 h)at a dose of 0,1,5,or 10 μg of 235U,respectively.The microautoradiographic tracing was performed,somatic growth and neurobehavior development of neonatal rats were examined by determination of multiple parameters,and the neuron specific enolase(NSE)and interleukin-1β(IL-1β) levels in brains were determined with radioimmunoassay. Results The radionuclides were mainly accumulated in the neuronal nucleus,and autoradiagraphic tracks appeared in the cytoplasm and inter cellular space.Neonatal rats showed delayed growth and abnormal neurobehavior.The changes of NSE,IL-1β in cerebellum,cerebral cortex,hippocampus,diencephalons showed a dose dependent relationship that when the dose of irradiation was increased,the levels of NSE was decreased and the IL-1β was increased. Conclusion The nerve cell of developing brain of neonatal rats issensitive, fragile and compensable to injurious effects of α irradiation from enriched uranium. |
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