| 吴丛梅,李修义,刘树铮.pcEgr-IFNγ基因治疗联合放射治疗抗肿瘤作用的实验研究[J].中华放射医学与防护杂志,2001,21(4):236-238.WU Congmei,LI Xiuyi,LIU Shuzheng.Experimentalal study on anti-tumor effect of pcEgr-IFNγ gene-radiotherapy[J].Chin J Radiol Med Prot,2001,21(4):236-238 |
| pcEgr-IFNγ基因治疗联合放射治疗抗肿瘤作用的实验研究 |
| Experimentalal study on anti-tumor effect of pcEgr-IFNγ gene-radiotherapy |
| 投稿时间：2001-04-30 |
| DOI： |
| 中文关键词: pcEgr-IFNγ重组质粒 X射线照射 B16黑色素瘤 基因放射联合治疗 |
| 英文关键词:pcEgr-IFNγ reconstruction plasmid X-ray irradiation B16 melanoma Gene-radiotherapy |
| 基金项目:国家自然科学基金资助项目(39970229) |
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| 中文摘要: |
| 目的 探讨pcEgr-IFNγ基因-放射联合治疗对移植黑色素瘤B16小鼠的抗肿瘤作用及其免疫学机理.方法 肿瘤局部注射脂质体包裹的pcEgr-IFNγ重组质粒后 36h,接受 2 0GyX射线照射,观察放疗后不同时间肿瘤大小,放疗后 3d瘤内IFNγ转录水平和 15d部分免疫学指标.结果pcEgr-IFNγ基因-放射联合治疗后第 3～15天,肿瘤生长速率明显低于单纯照射组,照后第 9～15天明显低于单纯基因治疗组和空质粒对照组;治疗后第 3天,pcEgr-IFNγ基因-放射联合治疗组瘤内IFNγ表达水平明显高于单纯基因治疗组;脾脏NK细胞毒活性、IL 2和IFNγ分泌活性均明显高于单纯放疗组和单纯基因治疗组.结论 pcEgr-IFNγ基因-放射联合治疗抗肿瘤作用明显优于单纯放疗和单纯基因治疗组,其机理可能与放疗诱导瘤内IFNγ基因表达增强,激活荷瘤机体抗肿瘤免疫功能有关. |
| 英文摘要: |
| Objective To study the anti-tumor effect of IFN-γ gene-radiotherapy to murine melanoma and its immunologic mechanism. Methods pcEgr-IFNγ plasmids were injected locally into tumor,and 36 hours later,the tumors were given 20 Gy X-ray irradiation.Tumor growth at different time,IFN-γ expression 3 days later and immunologic indexes 15 days later were detected. Results At 3-15 days after pcEgr-IFNγ gene-radiotherapy,the tumor growth rate was lower than that of irradiation alone group.It was also lower than that of gene thempy alone group and control plasmid combined with X-ray irradiation group significantly. Day 3 tumor IFN y expression was higher than that of plasmid treatment alone group. NK activity, IL-2 and IFN y secretion activities were higher than those of genetherapy alone and irradiation alone groups significantly. Conclusion The anti-tu-mor effect of IFN y gene-radiotherapy is better than that of either of them applied solely. Its mechanism might be conccerned with the higher expression of IFN y induced by irradiation in tumors and activation of anti-tumor immu-nologic functions. |
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