中华放射医学与防护杂志

庄永志,王俊杰,张占春,等.γ射线对大鼠血管平滑肌细胞抑制作用的机理[J].中华放射医学与防护杂志,2001,21(4):233-235.ZHUANG Yongzhi,WANG Junjie,ZHANG Zhanchum,et al.The mechanism of inhibitory effect of γ-ray irradiation on rat vascular smooth muscle cells[J].Chin J Radiol Med Prot,2001,21(4):233-235

γ射线对大鼠血管平滑肌细胞抑制作用的机理

The mechanism of inhibitory effect of γ-ray irradiation on rat vascular smooth muscle cells

投稿时间：2001-04-30

DOI：

中文关键词: VSMCs γ射线凋亡再狭窄

英文关键词:VSMCs γ-ray irradiation apopotosis restenosis

基金项目:国家自然科学基金资助项目(39770192)

作者	单位
庄永志	100083 北京大学第三医院肿瘤治疗中心
王俊杰	100083 北京大学第三医院肿瘤治疗中心
张占春	100083 北京大学第三医院肿瘤治疗中心
贾廷珍	100083 北京大学第三医院肿瘤治疗中心

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中文摘要:

目的探讨γ射线对大鼠血管平滑肌细胞 (VSMCs)抑制作用的机理.方法应用克隆形成实验绘制VSMCs剂量存活曲线,采用³H掺入法观察γ射线对VSMCs增殖的影响.应用流式细胞仪和"DNA Ladder"技术检测γ射线对VSMCs凋亡的影响.结果 VSMCs的D0 值为 1.95Gy,Dq值为 1.76Gy,D₃₇值为 3.71Gy,N值为 2.47.射线对VSMCs增殖的抑制作用呈剂量依赖性,随剂量增加而增大. 2、10、2 0Gy照射后 48h内VSMCs凋亡数量均未增加.结论 γ射线可抑制VSMCs增殖,其机理主要是γ射线对VSMCs中有克隆形成能力的细胞进行杀伤以及抑制其进行有丝分裂.

英文摘要:

Objective To investigate the inhibitory effect of γ-ray irradiation on rat vascular smooth muscle cells (VSMCs). Methods Dose-survival curve of VSMCs was figured by colony formation.The effect of γ-ray irradiation on viability and proliferation of VSMCs was observed by ³H incorporation.Flow cytometry and DNA Ladder were used to detect the apopotogenic effect of γ-ray irradiation on VSMCs. Results The values of D₀,Dq,D₃₇ and N for VSMCs were 1.95 Gy,1.76 Gy,3.71 Gy and 2.47, respectively.The inhibitory effect of γ-ray irradiation on VSMCs proliferation was dose-dependent,being stronger along with increase of dose.VSMCs did not undergo apoptosis within 48 hours after γ-ray irradiation. Conclusion γ-ray irradiation could inhibit the proliferation of VSMCs,the main mechnism of which is the killing effect and inhibition of mitosis of VSMCs.

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