| 庄永志,王俊杰,张占春,贾廷珍.γ射线对大鼠血管平滑肌细胞抑制作用的机理[J].中华放射医学与防护杂志,2001,21(4):233-235 |
| γ射线对大鼠血管平滑肌细胞抑制作用的机理 |
| The mechanism of inhibitory effect of γ-ray irradiation on rat vascular smooth muscle cells |
| 投稿时间：2001-04-30 |
| DOI： |
| 中文关键词: VSMCs γ射线 凋亡 再狭窄 |
| 英文关键词:VSMCs γ-ray irradiation apopotosis restenosis |
| 基金项目:国家自然科学基金资助项目(39770192) |
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| 中文摘要: |
| 目的 探讨γ射线对大鼠血管平滑肌细胞 (VSMCs)抑制作用的机理.方法 应用克隆形成实验绘制VSMCs剂量存活曲线,采用3H掺入法观察γ射线对VSMCs增殖的影响.应用流式细胞仪和"DNA Ladder"技术检测γ射线对VSMCs凋亡的影响.结果 VSMCs的D0 值为 1.95Gy,Dq值为 1.76Gy,D37值为 3.71Gy,N值为 2.47.射线对VSMCs增殖的抑制作用呈剂量依赖性,随剂量增加而增大. 2、10、2 0Gy照射后 48h内VSMCs凋亡数量均未增加.结论 γ射线可抑制VSMCs增殖,其机理主要是γ射线对VSMCs中有克隆形成能力的细胞进行杀伤以及抑制其进行有丝分裂. |
| 英文摘要: |
| Objective To investigate the inhibitory effect of γ-ray irradiation on rat vascular smooth muscle cells (VSMCs). Methods Dose-survival curve of VSMCs was figured by colony formation.The effect of γ-ray irradiation on viability and proliferation of VSMCs was observed by 3H incorporation.Flow cytometry and DNA Ladder were used to detect the apopotogenic effect of γ-ray irradiation on VSMCs. Results The values of D0,Dq,D37 and N for VSMCs were 1.95 Gy,1.76 Gy,3.71 Gy and 2.47, respectively.The inhibitory effect of γ-ray irradiation on VSMCs proliferation was dose-dependent,being stronger along with increase of dose.VSMCs did not undergo apoptosis within 48 hours after γ-ray irradiation. Conclusion γ-ray irradiation could inhibit the proliferation of VSMCs,the main mechnism of which is the killing effect and inhibition of mitosis of VSMCs. |
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