| 何佩,丁振华,郑莉,杨军,罗成基.放烧复合伤对小鼠白细胞介素-3的影响[J].中华放射医学与防护杂志,2001,21(3):192-194 |
| 放烧复合伤对小鼠白细胞介素-3的影响 |
| Effects of combined radiation-burn injury on the serum level of IL-3 in mice |
| 投稿时间：2000-08-04 |
| DOI： |
| 中文关键词: 放射损伤 烧伤 放烧复合伤 白细胞介素-3 小鼠 造血功能障碍 |
| 英文关键词:Irradiation Burn Combined radiation-burn injury Interleukin-3 Mice Hematopoietic failure |
| 基金项目:全军九五指令性课题资助项目(96L045) |
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| 中文摘要: |
| 目的 探讨放烧复合伤所致造血功能障碍与内源性IL-3之间的关系。方法 小鼠随机分为正常(N)、放射损伤(R)、烧伤(B)及放烧复合伤(C)共4组,利用EndogenMouseIL3ELISA试剂盒,检测小鼠致伤后不同时间的血清IL-3水平。结果 R组血清IL-3水平明显降低,其中第3、5天最低(低于检测灵敏度),第15天开始缓慢回升,第28天仍未达正常水平;B组第3、5天低于正常,第10天开始回升,第15天达高峰,为正常组的210%;C组血清IL3变化介于R、B组之间;各观测组外周血白细胞数量的变化与血清IL3变化呈平行关系。小鼠15%Ⅲ度烧伤后1～11d的血清对小鼠骨髓基质细胞(BMSC)贴壁率和CFU-F的形成主要表现为抑制作用,13～15d的血清主要表现为刺激作用。结论 小鼠烧伤后不同时间的血清对BMSC贴壁率和CFUF集落形成的作用不同以及放烧复合伤所致外周血白细胞数量异常可能与内源性IL-3的变化有关。本实验提示：内源性IL-3水平不足可能是放烧复合伤所致造血功能障碍形成的原因之一。 |
| 英文摘要: |
| Objective To explore the relationship between endogenous interleukin-3 and hematopoietic failure caused by combined radiation-burn injury. Methods Mice were randomized into normal (N),irradiation (R),burn (B) and combined irradiation-burn injury (C) groups.Serum level of IL-3 was detected by ELISA using the Endogenous Mouse IL-3 ELISA Kit at different times after injury. Results Compared with group N the serum level of IL-3 was significantly decreased in group R,especially on the 3rd and the 5th day (below the detecting sensitivity).It recovered slowly from the 15th day and did not elevate to the normal value on the 28th day.In group B.IL-3 was less than normal value on the 3rd and 5th day,then it increased rapidly and reached the peak (210% of that of the group N) on the 15th day.In group C,the serum level of IL-3 was higher than that of group R but lower than that of group B.The changes of the peripheral white blood cell in each group were parallel with the changes of IL-3.The serum of burnt mice collected from the 1st to 11 day showed inhibitory effect on CFU-F,stromal cell adhering rate of bone marrow stromal cells (BMSC),whereas serum harvested from the 13th to 15th days showed stimulation effect. Conclusion The serum of burnt mice collected at different times showing different actions on BMSC,and abnomal WBC caused by combined radiation-burn injury might be related with the changes in endogenous IL-3.The results of this experiment indicates:inhibition of endogenous IL-3 is one of the causes of hematopoietic failure induced by combined radiation-burn injury in mice. |
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