| 王延江,粟永萍,艾国平,冉新泽,刘晓宏,袁良平,程天民.全身辐射对小鼠巨噬细胞IL-12表达影响的实验研究[J].中华放射医学与防护杂志,2001,21(2):96-99 |
| 全身辐射对小鼠巨噬细胞IL-12表达影响的实验研究 |
| Influence of total body irradiation on IL-12 expression in murine macrophages |
| 投稿时间：2000-08-20 |
| DOI： |
| 中文关键词: 辐射 细胞免疫 巨噬细胞 IL-12 |
| 英文关键词:Radiation Cell mediated immunity Macrophage IL-12 |
| 基金项目:全军九五攻关课题基金资助项目(96L045) |
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| 中文摘要: |
| 目的 探讨γ射线全身照射对小鼠腹腔巨噬细胞数量、形态和IL-12基因表达影响的时效和量效特点,为放射损伤后的免疫调控提供理论依据。方法 小鼠一次性全身照射,于不同时相点分离、计数腹腔巨噬细胞,并观察其形态学变化,RT-PCR法检测其IL-12p35和p40的基因转录水平。结果 ①伤后巨噬细胞数量早期减少,后期恢复；②伤后巨噬细胞呈多形性和纤维状；③伤后IL-12p35转录水平降低,而p40转录水平增加；④照射剂量越大,巨噬细胞损伤效应越重,恢复越慢。伤后7d内以损伤改变为主,15d后以再生修复、功能恢复为主。结论 全身照射后巨噬细胞数量减少,形态改变,以及IL-12p35的减少和p40/p40的增加,可能是免疫障碍的重要环节。只有同时检测IL-12p35和p40才能正确反映放射损伤后IL-12的基因表达状态 |
| 英文摘要: |
| Objective To investigate the influence of total body irradiation(TBI)on the cell count,morphology and IL-12 transcriptive level of peritoneal macrophages(pM¢s). Methods At different timepoints post TBI,pM¢s were purified,counted and observed under microscope,and the transcriptive level of IL-12 subunits was detected with RT PCR. Results After irradiation:①pM¢s decreased on days 3 and 7,then partially recoveved on days 15 and 30;②pM¢s looked like polymorphic and fibriform;③IL-12 p35 transcription was suppressed while that of p40 was elevated;④the higher the dose,the more serious the damage and the slower its recovery.The damage was predominant within 7 days,while the recovery become evident beginning from day 15 after radiation. Conclusion Reduction and transfiguration of pM¢s,suppression of IL-12 p35 transcription and elevation of IL-12 p40 transcription might be important contributors of immunity suppression.Only combination of p35 transcription with that of p40 should be used appropriately to evaluate the state of IL-12 expression after irradiation. |
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