| 楼铁柱,葛世丽,项晓琼,吴德昌.α粒子诱发转化人支气管上皮细胞BEP2D中p53基因的突变特点[J].中华放射医学与防护杂志,2001,21(2):93-95 |
| α粒子诱发转化人支气管上皮细胞BEP2D中p53基因的突变特点 |
| Mutation of p53 gene in human bronchial epithelial cells BEP2D induced by α-particles |
| 投稿时间：2000-07-11 |
| DOI： |
| 中文关键词: α粒子 BEP2D 细胞转化 p53 聚合酶链式反应-单链构象多态性分析 |
| 英文关键词:α-particles BEP2D Cell transformation p53 PCRSSCP |
| 基金项目:国家重点基础研究专项经费资助项目(G1998051200) |
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| 中文摘要: |
| 目的 观察α粒子诱发BEP2D细胞转化过程中p53基因的改变。方法 聚合酶链式反应单链构象多态性分析(PCR-SSCP).结果 分析发现在细胞转化过程中,p53基因发生重要改变,照射后早期传代细胞中,p53外显子7就发生碱基突变,经酶切分析为249密码子的改变,外显子5/6在照后20代细胞内开始丢失,并经Southern杂交证实,以上改变均在转化过程中持续存在；而外显子8/9未见变化。结论 p53基因外显子5、6、7是α粒子对DNA损伤的重要靶位点,在α粒子诱发支气管上皮细胞转化的过程中发挥重要作用。 |
| 英文摘要: |
| Objective To detect p53 gene mutation in BEP2D cells induced by α-particles.Methods Analysis of genomic DNA from the transformed cells using PCR amplification and single strand conformation polymorphism (SSCP).Results SSCP and restriction enzyme analysis demonstrated a point mutation at codon 249 of tumor suppressor gene p53 which has been thought to be the “hotspot” of mutation in transformation induced by α particles.Southern blot analysis showed a deletion in exon 5/6 of p53 gene,but there was no mutation in the exon 8/9 of p53 gene.Conclusion p53 gene is the “target” of DNA lesions induced by α particles.It plays an important role in the process of transformation of bronchial epithelial cells induced by α irradiation. |
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