| 王晓琳,李进,王知权.用荧光原位杂交技术建立60Coγ射线诱发人外周血淋巴细胞染色体易位率剂量效应曲线及易位的持续性研究[J].中华放射医学与防护杂志,2001,21(2):83-86 |
| 用荧光原位杂交技术建立60Coγ射线诱发人外周血淋巴细胞染色体易位率剂量效应曲线及易位的持续性研究 |
| Fluorescence in situ hybridization detection of chromosome translocations induced by 60Coγ-rays in human lymphocytes dose-response curve and persistence |
| 投稿时间：2000-04-20 |
| DOI： |
| 中文关键词: 60Co源辐射 荧光原位杂交 易位 剂量效应曲线 持续性 |
| 英文关键词:60Co γ-radiation Fluorescence in situ hybridization Translocation Doseresponse curve Persistence |
| 基金项目:中日协作课题;院校科研基金资助项目(952060) |
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| 中文摘要: |
| 目的 研究不同剂量60Coγ射线诱发人外周血淋巴细胞染色体易位率和辐射剂量间的剂量效应关系以及观察易位率在同一剂量、不同时相点的变化。方法 采用荧光原位杂交技术和连续Giemsa法,用4号和7号全染色体探针分析0、0.25、0.5、0.75、1和2Gy60Coγ射线离体照射诱发人外周血淋巴细胞染色体易位率,同时对两个剂量点进行52和72h培养,比较其易位率。结果 易位率和辐射剂量间的量效关系可以用一个二次多项式方程Y=0.0030+0.0134D+0.0165D2来描述；易位率在52和72h培养时差异无显着性。结论 易位率和辐射剂量间存在良好的量效关系,可作为进行早先照射剂量重建的理论依据 |
| 英文摘要: |
| Objective To study doseresponse relationship for chromosome translocation and its persistence measured in human lymphocytes exposed to 60Co γ-rays.Methods The chromosome translocations in human peripheral lymphocytes were detected by fluorescence in situ hybridization using 4# and 7# combination of composite whole chromosomespecific DNA probes and Giemsa stain.Results The doseresponse relationship for chromosome translocation induced by 60Co γ-rays in vitro could be described by the function:Y=0.0030+0.0134D+0.0165D2.The frequencies of chromosome translocations induced by 0.5 and 2 Gy γirradiation did not diminish over time,so it exhibited excellent persistence.Conclusions The results indicate that retrospective dosereconstruction can be accomplished using chromosome translocation frequency. |
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