| 苏炳银,蔡文琴,张成岗,等.60Coγ射线全身照射后大鼠脑GAP-43 mRNA的表达增强[J].中华放射医学与防护杂志,2001,21(2):80-82.SU Bingyin,CAI Wenqin,ZHANG Chenggang,et al.60Coγirradiation enhances expression of GAP-43 mRNA in rat brain[J].Chin J Radiol Med Prot,2001,21(2):80-82 |
| 60Coγ射线全身照射后大鼠脑GAP-43 mRNA的表达增强 |
| 60Coγirradiation enhances expression of GAP-43 mRNA in rat brain |
| 投稿时间：2000-05-20 |
| DOI： |
| 中文关键词: 60Coγ射线 GAP-43 mRNA 大鼠 脑 神经再生 |
| 英文关键词:60Co γ-rays GAP43mRNA Rat Brain Nerve regeneration |
| 基金项目:国家自然科学基金资助项目(39900078);全军95科研基金;重庆市应用基础研究课题 |
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| 中文摘要: |
| 目的 研究60Coγ射线照射后大鼠脑GAP-43表达的变化与神经再生的关系。方法 地高辛标记的cRNA探针原位杂交。结果 正常对照大鼠除海马锥体细胞层有较强的阳性标记外,其他脑区仅有弥散的GAP-43mRNA弱阳性标记。8Gy60Coγ射线照射1周后,大脑皮质、基底核、丘脑和下丘脑的大部分核团GAP-43呈中等强度的表达,照射后第4周,以上脑区的GAP-43mRNA水平明显升高,第7周GAP-43的表达逐渐减弱,但仍高于正常水平。结论 60Coγ射线照射致大鼠脑损伤后GAP-43mRNA表达增强,可能与损伤神经元结构再生和突触重建过程密切相关。 |
| 英文摘要: |
| Objective To study the relationship between the expression of GAP-43 mRNA and nerve regeneration in rat brain after 60Co γ-irradiation. Methods Wistar rats were subjected to wholebody irradiation with 8 Gy 60Co γ-rays.The expression of GAP-43 was detected by in situ hybridization histochemistry using DigcRNA probe. Results It was found that the expression of GAP-43 mRNA increased in the cerebral cortex,caudate,putamen,globus pallidus,thalamus and hypothalamus one week after 8 Gy 60Co γ-irradiation.The peak of GAP-43 mRNA expression was observed in the fourth week and then began to decrease but still remained at a higher than normal level.However,it decreased to a low level after 7 weeks. Conclusion Enhanced expression of GAP-43 mRNA after 60Co γ-irradiation in rat brain is associated with nerve regeneration and reconstruction of synapse. |
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