| 项莺松,杨如俊,唐古生,许熊飞,张明辉,刘书逊,蔡建明,杨平,曹雪涛.不同剂量γ射线照射后胎儿骨髓CD34+造血干细胞死亡规律[J].中华放射医学与防护杂志,2001,21(2):73-75 |
| 不同剂量γ射线照射后胎儿骨髓CD34+造血干细胞死亡规律 |
| Characterization of death of human fetal bone marrow CD34+ cells after different dose of γ-irradiation |
| 投稿时间：2000-08-25 |
| DOI： |
| 中文关键词: 造血干细胞 凋亡 增殖期死亡 龛位 |
| 英文关键词:Hematopoietic stem cell apoptosis reproductive death niche |
| 基金项目:国家自然科学基金资助项目(39770329,39600077) |
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| 中文摘要: |
| 目的 探讨不同剂量γ射线照射后胎儿骨髓CD34+造血干细胞的死亡规律。方法 以流式细胞术PE-CD34+FITC-AnnexinV/7AAD三色荧光法,多参数分析了受不同剂量γ射线照射后,不同时间点的胎儿骨髓CD34+造血干细胞凋亡、坏死和存活的时效和量效变化特点。结果 受大剂量率γ射线1次5Gy和8Gy照射后,胎儿骨髓CD34+造血干细胞的死亡是在照射后为期1周的连续性死亡,既有凋亡又有坏死,但是以凋亡为主；受大剂量率γ射线1次10Gy和12Gy照射后,胎儿骨髓CD34+造血干细胞则在受照后当天即大量坏死,在持续1周内全部死亡。结论 受大剂量率γ射线1次照射后,造血干细胞在1周内发生了增殖期死亡而连续性空出所占据的龛位。 |
| 英文摘要: |
| Objective To investigate the characterization of death of the human hematopoietic stem cells after irradiation. Methods Human fetal bone marrow mononuclear cells were irradiated with different doses of 60Co γ-rays at different high dose rates.Apoptosis and necrosis of CD34+ cells were analyzed by flow cytometry,following threecolor labeling with PE-CD34/FITC-Annexin V/7AAD at different times after irradiation. Results The death of CD34+ cells after 5 Gy and 8 Gy irradiation showed a continuous process of reproductive death during the first week,and the main death type was apoptosis.A majority of CD34+ cells died of necrosis during the first day after 10 Gy and 12 Gy irradiation,and all of them died within a week. Conclusion Niches are continuously vacated every day within a week following irradiation and reproductive death of hematopoietic stem cells occurred. |
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