| 彭瑞云,王德文,熊呈琦,高亚兵,杨红,崔玉芳,汪宝珍.辐射诱导骨髓细胞凋亡及相关基因表达研究[J].中华放射医学与防护杂志,2001,21(1):19-20 |
| 辐射诱导骨髓细胞凋亡及相关基因表达研究 |
| Studies on hematopoietic cell apoptosis and the relative gene expression in irradiated mouse bone marrow |
| 投稿时间：2000-03-18 |
| DOI： |
| 中文关键词: 辐射 骨髓 凋亡 bcl2 p53 |
| 英文关键词:Radiation Bone marrow Apoptosis bcl2 p |
| 基金项目:全军“九五”青年基金资助项目(98Q081) |
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| 中文摘要: |
| 目的研究辐射诱导骨髓细胞凋亡、bcl-2和p53基因表达及意义。方法γ射线全身照射小鼠,采用原位末端标记、原位杂交和图像分析等技术,研究骨髓细胞凋亡及bcl-2和p53表达。结果照射后6h骨髓细胞凋亡；bcl-2于细胞凋亡时减少,而于其后修复早期增加；p53于造血细胞凋亡及之后修复均增多,而mtp53仅于其修复时阳性。结论辐射可导致骨髓细胞凋亡,且呈剂量相关性；bcl-2与p53基因均参与其凋亡和修复过程。 |
| 英文摘要: |
| Objective To study apoptosis and expressions of bcl-2 and p53 in irradiated mouse bone marrow. Methods LACA mice were irradiated with 60 Co γ rays.By means of in situ terminal labeling, in situ hybridization and image analysis,the authors studied radiation-induced apoptosis of hematopoietic cells and the expressions of bcl-2 and p53. Results The characteristics of apoptosis appeared in hematopoietic cells at 6 hrs after irradiation.The expression of bcl-2 was obviously decreased when apoptosis of hematopoietic cells occurred,whereas it increased in the early recovery phasep53 protein increased during both apoptosis of hematopoietic cells and the recovery phase,and mutant type p53 DNA was positive only in the recovery phase. Conclusion Radiation may induced apoptosis of hematopoietic cells in a dose-dependent mannerBoth bcl-2 and p53 genes play an important role in apoptosis and recovery phase. |
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