| 马增春,高月,刘永学,谭洪玲,张立,陶来宝,陈鹏.辐射小鼠骨髓CD34+细胞的变化及其意义[J].中华放射医学与防护杂志,2001,21(1):12-15 |
| 辐射小鼠骨髓CD34+细胞的变化及其意义 |
| The changes of CD34+ cells in C57 mouse bone marrow after irradiation and their roles in dysfunction of hematopoiesis |
| 投稿时间：2000-05-15 |
| DOI： |
| 中文关键词: 辐射 骨髓 细胞凋亡 CD34 细胞周期 |
| 英文关键词:Irradiation Bone marrow Apoptosis CD34 Cell cyclX |
| 基金项目:国家自然科学基金资助项目(39770887) |
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| 中文摘要: |
| 目的研究γ射线照射后C57小鼠骨髓中CD34+细胞的数量变化规律及其意义。方法流式细胞仪测定CD34+结果①CD34+细胞在骨髓有核细胞中的比例随照射剂量的加大而降低,在55Gy照射后14d内小鼠CD34+细胞的减少表现为持续性；②小鼠照射后6h骨髓细胞凋亡率最高,以5.5Gy照射组最为明显；③5.5Gy照射后小鼠骨髓细胞周期紊乱。结论γ射线损伤骨髓中的干祖细胞,造成骨髓中干祖细胞的数量减少,其途径之一是诱导骨髓细胞凋亡。 |
| 英文摘要: |
| Objective To observe the changes of CD34 + cells in C57 mouse bone marrow after irradiation and investigate the role of apoptosis in radiation-induced dysfunction of hematopoiesis. Methods Flow cytometric enumeration of CD34 + hematopietic stem and progenitor cells by double fluorescent labeling apoptosis detection by Annexin V-FITC kit,and cell cycle detection by PI labeling were carried out. Results ①Compared with the normal group,the percentage of CD34 + cells in bone marrow nucleated cells decreased at least for 14 days after irradiation,and the changes were related with irradiation doses.②At 6 h after irradiation,the largest amount of apoptopic cells could be detected.③Bone marrow cell cycle was perturbed after 5.5 Gy irradiation. Conclusion The percentage of CD34+ hematopietic stem and progenitor cells in C57 mouse bone marrow decreased after irradiation,and apoptosis might be responsible for the changes of the bone marrow cells. |
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