| 郭德煌,董波,刘农乐,等.ras-raf信号转导途径对受辐射细胞G1期阻滞的调控作用[J].中华放射医学与防护杂志,2000,20(6):408-410.GUO Dehuang,DONG Bo,LIU Nongle,et al.The regulation of ras-raf signaling pathway on G1 phase of the irradiated cells[J].Chin J Radiol Med Prot,2000,20(6):408-410 |
| ras-raf信号转导途径对受辐射细胞G1期阻滞的调控作用 |
| The regulation of ras-raf signaling pathway on G1 phase of the irradiated cells |
| 投稿时间：2000-04-04 |
| DOI： |
| 中文关键词: 辐射 细胞周期 周期蛋白D1 |
| 英文关键词:Irradiation Cell cycle Cyclin |
| 基金项目:军事医学科学院博士后基金资助项目(20101) |
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| 中文摘要: |
| 目的 探讨rasraf信号转导途径在受辐射KG-1细胞中对周期的调控作用方式。方法 通过转染DN-ras阻断GMCSF的信号传递后,瞬间转染cyclinD1,观察cyclinD1对受辐射细胞周期阻滞的影响作用。结果 转染DN-ras的受辐射KG-1细胞即使用GMCSF刺激亦不能跳出G1期阻滞;瞬间转染cyclinD1能促进受辐射细胞跳出周期阻滞。结论 rasraf信号转导途径通过促进周期蛋白cyclinD1的表达而对受辐射细胞周期进行调控。 |
| 英文摘要: |
| Objective To investigate the way of ras-raf signaling pathway which regulate the G1 phase in irradiated KG-1 cells. Methods Blocked the GM-CSF signaling pathway by transfected DN-ras and then momentary transfected cyclin D1 into irradiated KG-1 cells, the effects of cyclin D1 on G1 phase was examined. Results The irradiated KG-1 cells transfected DN-ras can't recover form G1 phase arrest even though the GM-CSF was given, momentary transfected cyclin D1 promote the irradiated KG-1 cells from G1 arrest. Conclusion Activation of ras-raf signaling pathway regulate the cell cycle of the irradiated KG-1 cells through promotion the expression of the cyclin D1. |
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