| 刘纯杰,王德文,高亚兵,等.细胞因子及其受体在放射性肺纤维化发生中的分子病理机理研究[J].中华放射医学与防护杂志,2000,20(6):391-394.LIU Chunjie,WANG Dewen,GAO Yabing,et al.Role of growth factors in molecular pathogenetic mechanism of radiation pulmonary fibrosis[J].Chin J Radiol Med Prot,2000,20(6):391-394 |
| 细胞因子及其受体在放射性肺纤维化发生中的分子病理机理研究 |
| Role of growth factors in molecular pathogenetic mechanism of radiation pulmonary fibrosis |
| 投稿时间：1999-11-12 |
| DOI： |
| 中文关键词: 细胞因子 受体 分子病理机理 放射性肺纤维化 |
| 英文关键词:Cytokine Receptor Molecular pathogenetic mechanism Radiation pulmonary fibrosis |
| 基金项目:全军九五指令基金资助项目(96L011) |
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| 中文摘要: |
| 目的 调查几种细胞因子及其受体在放射性肺纤维化(RPF)发生中的作用。方法 采用免疫组织化学、细胞免疫化学和原位杂交的方法对RPF大鼠肺组织细胞进行了研究。结果 正常大鼠肺组织有TGFβ1和TGFβR的弱表达,照射动物肺脏TGFβ1的表达于照射后2周开始增高,从第8周直到3月表达水平最高。肺脏表达TGFβ1的部位主要是支气管上皮细胞、肺泡巨噬细胞、肺泡上皮细胞、支气管和血管平滑肌细胞以及成纤维细胞等。TGFβ2的表达变化规律除了比TGFβ1稍弱外类似于TGFβ1.TGFβR表达增强的时间比TGFβ1稍晚,于第8周开始增强,直到1年也保持较高水平的表达。bFGF和PDGF在照射后2～3个月表达水平明显增高。对支气管肺泡灌洗液中的细胞TGFβ1免疫细胞化学研究表明:其中的细胞,特别是巨噬细胞表达时间出现得较早,于照射后1周即有许多巨噬细胞呈现TGFβ1较强的表达。从肺组织培养的细胞研究表明,来源于照射后3月肺组织的成纤维细胞呈现较强的TGFβ1阳性。用地高辛标记的TGFβ1探针和α1(I)寡核苷酸探针原位杂交试验表明,TGFβ1mRNA表达水平于2～8周表达最高,而α1(I)前胶原mRNA则从6周开始增强,到3月表达出现高峰,稍滞后于TGFβ1的峰值期,与TGFβ1的表达呈现明显的时空关联和交叠现象。结论 TGFβ1在RPF发生中起着重要的作用,TGFβ2、TGFβR、bFGF和PDGF-A也参与了RPF的形成过程。 |
| 英文摘要: |
| Objective To investigate the role of growth factors and their receptors in radiation pulmonary fibrosis(RPF). Methods Immunohistochemisty, immunocytochemistry and in situ hybridization were used. Results The normal rat lung tissue weakly expressed TGFβ1 and TGFβ receptor (TGFβR).The expression of TGFβ1 in rat lung increased at 2 weeks after irradiation and its highest level maintained from 8 weeks to 3 months.The positive localization of TGFβ1 in lung was the epithelial cells of bronchi, alveolar macrophages, alveolar epithelial cells, smooth muscle cells of the bronchial and arteriolar wall and fibroblasts.The expression of TGFβ2 was similar to that of TGFβ1.The time of increased expression of TGFβR was later than that of TGFβ1.i, e. It increased at 8 weeks and kept a higher level of expression throughout one year.Stronger expressions of the bFGF and PDGF were also observed in 2-3 months postirradiation.The expression of TGFβ1 in the cells of bronchoalveolar lavage fluid was investigated.The results showed that macrophages were one of the earliest cells showing positive reaction, i, e. they presented positive at 1 week. For the cultured Wistar rat lung fibroblasts, TGFβ1 expression was stronger at 3 months postirradiation. By means of in situ hybridization with TGFβ1 probe and α1(I) oligonucleotide probe, the expression of TGFβ1 mRNA was increased at 2-8 weeks and α1(I) procollagen mRAN was increased at 6 weeks, but the expression peak appeared at 3 months postirradiation. The expressions of TGFβ1 and α1(I) procollagen were mutually connected and overlapped both in time and space. Conclusion TGFβ1 may play an important role in the pathogenesis of RPF. TGFβ2, TGFβR, bFGF and PDGF-A also participate in the pathogenetic process. |
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