中华放射医学与防护杂志

陈晓红,程天民,冉新泽.电离辐射对大鼠伤口中性粒细胞肌动蛋白的影响[J].中华放射医学与防护杂志,2000,20(6):388-390.CHEN Xiaohong,CHENG Tianmin,RAN Xinze.Effects of irradiation on actin in wound neutrophils of rats[J].Chin J Radiol Med Prot,2000,20(6):388-390

电离辐射对大鼠伤口中性粒细胞肌动蛋白的影响

Effects of irradiation on actin in wound neutrophils of rats

投稿时间：1999-09-08

DOI：

中文关键词: 放射复合伤中性粒细胞肌动蛋白

英文关键词:Wound combined with radiation injury Neutrophils Actin

基金项目:重庆市科委院士基金资助项目(985081);国家重点基础研究973专项经费资助项目(G1999054205)

作者	单位
陈晓红	400038 重庆, 第三军医大学中国人民解放军复合伤研究所
程天民	400038 重庆, 第三军医大学中国人民解放军复合伤研究所
冉新泽	400038 重庆, 第三军医大学中国人民解放军复合伤研究所

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全文下载次数: 2017

中文摘要:

目的从伤口早期中性粒细胞(Neu)肌动蛋白(actin)的变化方面探讨全身γ射线照射后Neu吞噬、趋化功能变化的机理及促愈药康复新(W_11^-a12)的作用机理。方法以大鼠6Gy全身照射复合皮肤切割伤为模型,收集伤口细胞。用Fluorescein鬼笔环肽标记Neu F-actin进行测定和观察。结果复合伤组Neu F-actin含量先高后低,对FMLP反应性减弱,FMLP刺激后解聚慢,康复新能改善Neu F-actin的功能状态。结论电离辐射引起Neu肌动蛋白功能异常可能是Neu趋化、吞噬活性降低的重要原因,改善Neu F-actin的功能状态是康复新提高Neu吞噬和趋化功能的作用机理。

英文摘要:

Objective To explore the mechanism of decreased phagocyotic and chemotactic function of neutrophils (Neu) and the mechanism of W_11^-a12' s pharmacological action by studying the changes of actin of Neu in wound after total body irradiation of rats. Methods Cells were collected from incision wounds on back of rats after 6 Gy whole body-irradiation.F-actin in Neu was stained with Fluorescein-phalloidin. Results There were increased (3～12 h) and decreased (24 h, 48 h) basal levels of F-actin, a lower responsiveness to stimulation and a reduced rate of actin depolymerization after combined radiation injury compared with simple wound group, which were important causes of Neu dysfunction. Conclusion W_11^-a12 could improve the functional state of Neu actin, which may contribute to the improved chemotactic and phagocytotic function of Neu after administration of this drug.

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