中华放射医学与防护杂志

傅海青,鞠桂芝,罗灿,等.p21在电离辐射诱导EL-4细胞G₁期阻滞中的作用[J].中华放射医学与防护杂志,2000,20(6):385-387.FU Haiqing,JU Guizhi,LUO Can,et al.The role of p21 in G₁ arrest of EL-4 cells induced by ionizing radiation[J].Chin J Radiol Med Prot,2000,20(6):385-387

p21在电离辐射诱导EL-4细胞G₁期阻滞中的作用

The role of p21 in G₁ arrest of EL-4 cells induced by ionizing radiation

投稿时间：1999-11-12

DOI：

中文关键词: 电离辐射 G1期阻滞 p21

英文关键词:Ionizing radiation G₁ arrest p21

基金项目:国家自然科学基金资助项目(39770193)

作者	单位
傅海青	130021 长春, 白求恩医科大学卫生部放射生物学重点实验室
鞠桂芝	130021 长春, 白求恩医科大学卫生部放射生物学重点实验室
罗灿	130021 长春, 白求恩医科大学卫生部放射生物学重点实验室
傅士波	130021 长春, 白求恩医科大学卫生部放射生物学重点实验室

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中文摘要:

目的探讨p21在电离辐射诱导EL-4细胞G₁期阻滞中的作用。方法采用Northernblot检测p21WAF1mRNA水平的变化;采用流式细胞术检测p21蛋白表达及细胞周期的变化。结果 4.0GyX射线照射后12h、24h、48hG₁期EL-4细胞百分数明显高于假照射组;p21WAF1mRNA水平从照射后1h开始升高,4h达峰值,持续至照后12h;p21蛋白表达在照射后2～48h明显增高。结论 4.0GyX射线照射可诱导EL-4细胞G₁期阻滞,p21在电离辐射诱导EL-4细胞G₁期阻滞中起重要作用。

英文摘要:

Objective To elucidate the role of p21 in G₁ arrest of EL-4 cells induced by ionizing radiation. Methods Northern blot was used to measure p21 WAF1 mRNA level.Flow cytometry was employed to analyze p21 protein expression and cell cycle of EL-4 cells. Results Following 4.0 Gy X-irradiation, EL-4 cells in G₁ phase began to significantly increase at 12 h, reached the peak at 24 h, and kept at high level till 48 h;the p21 WAF1 mRNA level began to increase at 1 h, reached the peak level at 4 h, kept the high levels till 12 h, then recovered to normal at 24 h;and the level of p21 protein expression in EI-4 cells increased significantly at 2 h to 48 h. Conclusion The results suggest that p21 might play an important role in G₁ arrest of EL-4 cells induced by ionizing radiation.

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