| 朱波,罗成基,郭朝华,等.60Coγ射线照射后培养小鼠骨髓基质细胞内NF-κB的变化[J].中华放射医学与防护杂志,2000,20(5):317-320.ZHU Bo,LUO Chengji,GUO Chaohua,et al.Changes of nuclear factor-κB (NF-κB) in cultured bone marrow stromal cells after 60Coγ-irradiation[J].Chin J Radiol Med Prot,2000,20(5):317-320 |
| 60Coγ射线照射后培养小鼠骨髓基质细胞内NF-κB的变化 |
| Changes of nuclear factor-κB (NF-κB) in cultured bone marrow stromal cells after 60Coγ-irradiation |
| 投稿时间：1999-12-24 |
| DOI： |
| 中文关键词: 骨髓基质细胞 辐射 骨髓造血微环境 NFκB |
| 英文关键词:Bone marrow stromal cells Radiation Bone marrow hematopoietic microenvironment NF-κB |
| 基金项目:全军“九五”指令性公关资助项目(P6L045) |
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| 中文摘要: |
| 目的 探讨辐射对培养小鼠骨髓基质细胞内NFκB的影响。方法 采用免疫组化、Westernblot及凝胶电泳迁移率实验。结果 免疫组化方法显示正常骨髓基质细胞细胞核的周围表达低水平的NF-κB,8.0Gy60Coγ射线损伤后1h即见其表达较正常显着升高并出现明显的核移位, 4h达高峰, 6h有所回落, 8h恢复正常；Westernblot显示照射后骨髓基质细胞NF-κB的蛋白表达量较正常明显升高, 8h仍然未恢复正常；凝胶电泳迁移率实验发现正常骨髓基质细胞内存在低水平活性NF-κB,8.0Gy60Coγ射线损伤后1h即见其活性明显升高, 4h达高峰, 8h恢复正常。结论 8.0Gyγ射线损伤后培养小鼠骨髓基质细胞内NF-κB的表达升高并激活, 这可能与骨髓微环境内基质细胞的抗辐射能力及造血功能的恢复有关。 |
| 英文摘要: |
| Objective To explore the changes in bone marrow hematopoietic microenvironment after exposure to γ-radiation. Methods Immunohistochemistry,Western blot and electrophoretic mobility shift assay (EMSA)was used. Results The nuclear translocation was found in cultured bone marrow stromal cells after 60 Coγ-irradiation with immunohistochemistry.The expression of NF- κB in the bone marrow stromal cells (BMSCs) on the level of protein was elevated after exposure to 60 Coγ-rays at the dose of 8. 0Gy with the uses of immunohistochemistry and Western blot.The activities of NF-κB in the BMSCs increased significantly after exposure to γ-radiation by using EMSA.The activity peak was at 4 hours after irradiation. Conclusion Our results suggest that the activation of NF-κB in the BMSCs by irradiation might be involved in the protection of BMSCs and in the recovery of hematopoiesis after γ-irradiation. |
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