| 王继先,于文儒,李本孝,樊体强,李征,高智伟,陈振军,赵永成.医用诊断X射线工作者GPA基因突变频率与累积受照剂量关系的研究[J].中华放射医学与防护杂志,2000,20(4):282-285 |
| 医用诊断X射线工作者GPA基因突变频率与累积受照剂量关系的研究 |
| Relationship between mutation frequency of GPA locus and cumulative dose among medical diagnostic X-ray workers |
| 投稿时间：1999-09-18 |
| DOI： |
| 中文关键词: 医用诊断X射线工作者 GPA基因突变检测 生物剂量计 |
| 英文关键词:Medical diagnostic X-ray workers GPA focus mutation assay Biodosimeter |
| 基金项目:国家自然科学基金资助项目(39470228) |
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| 中文摘要: |
| 目的 探讨GPA基因突变频率检测作为累积受照生物剂量计的可行性。方法 用改进的GPA基因位点突变检测技术, 分析了55名不同时期开始X射线工作者和50名非放射科医务工作者外周血红细胞GPA基因突变变异体频率与开始工作时期、工龄和累积受照剂量间的关系。结果 医用X射线工作者的GPA基因突变频率明显高于对照;突变率明显增高是发生在1970年前开始X射线工作者, 这与肿瘤流行病学调查所见恶性肿瘤危险明显增高发生在1970年前开始X射线工作者相一致;X射线工作者的GPA基因突变频率随累积剂量而增高, 剂量效应关系N突变优于NN突变。结论 用GPA基因突变检测作为生物剂量计, 还有许多问题需要解决, 如:个体差异、特异性及剂量效应刻度曲线等。 |
| 英文摘要: |
| Objective To explore the feasibility of using GPAlocus mutation assay as a biodosimeter for occupational exposure to ionizing radiation. Methods An improved technique of GPAlocus mutation assay was used in the study.The frequencies of mutant RBCin peripheral blood of 55 medical Xray workers and 50 controls employed in different calendar-year periods were detected.The relationship between mutation frequencies (MFs) and period of entry, working years and cumulative doses were analyzed. Results The MFs were significantly elevated among Xray workers employed before 1970.This finding is similar to the result of cancer epidemiological study among medical Xray workers, in which the cancer risk was significantly increased only Xray workers employed before 1970.The MFs of GPAincreased with increasing cumulative dose.The dose-effect relationship of NΦMF with cumulative dose was closer than that of NΦMF. Conclusion There are many problems to be solved for using GPA MF assay as a biodosimeter such as individual variation, specificity and calibration curve of dose-effect relationship. |
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