| 刘永葆,饶用清,许鹿希.60Coγ射线对成年小鼠脑内海马区组织形态上的影响[J].中华放射医学与防护杂志,2000,20(4):267-270 |
| 60Coγ射线对成年小鼠脑内海马区组织形态上的影响 |
| Effects of 60Coγ-radiation on brain hippocampal tissue of adult mice |
| 投稿时间：1999-10-13 |
| DOI： |
| 中文关键词: 海马 小鼠 电离辐射 神经病理学 |
| 英文关键词:Hippocampus Mice Ionizing radiation Neuropathology |
| 基金项目: |
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| 中文摘要: |
| 目的 探讨电离辐射对成年动物海马组织损伤的特点。方法 选用昆明品系雄性小白鼠, 用60Coγ射线对照射组进行全身均匀分次照射, 隔日1次, 每次2.0Gy, 从存活小鼠及对照组各随机取8只小鼠, 分别取脑, 石蜡包埋, 连续地平切片, Nissl和Weil染色及图像分析测量。结果 照射组海马的锥体层中的神经元数量较正常组减少, 神经元的主要病理改变是细胞体积变小, 核固缩, 核溶解, 照射组海马辐射层的神经纤维的面密度明显小于对照组, 两组之间差异有非常显着性(P<0.01).海马的4个亚区的辐射敏感性依次为CA1>CA2>CA3>CA4.结论 海马神经元的损伤可能是临床上放疗后发生记忆和学习能力减退等神经心理功能障碍的病理学基础 |
| 英文摘要: |
| Objective To study neuropathological changes of hippocampus tissue in adult mice following a series of irradiation with 60Co γ-rays.Methods Male mice of Kunming strain in experimental group( n =8)were exposed total bodily to 60Co γ-rays at 2.0 Gy once every two days. Ahistopathological imaging analysis of the mouse brain tissue was carried out after paraffin embedding and a series of sections were made and stained with Nissl and Weil staining methods. Results In the irradiation group (the cumulative dose=26 Gy) loss of pyramidal cells in hippocampus was significant when compared with the control group. Neuropathological changes were characterised by reduced neuron size, nuclear pyknosis and karyolysis. The neurofibrillar density of the pyramidal layer in the irradiation group was much lower than that of the control group ( P<001).Susceptibilities of the four subzones to the radiation appeared following order of CA1>CA2>CA3>CA4 in the hippocampus.Conclusion The neuronal damage in hippocampus after 60Co irradiation could form a pathological basis in reduction of memorial and learning ability. |
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