| 张雁云,张学光,张毅,王江方,邱玉华,刘蓓岭,谢炜,蒋冶棠.肿瘤坏死因子α对小鼠骨髓型放射损伤的防护作用[J].中华放射医学与防护杂志,2000,20(4):257-260 |
| 肿瘤坏死因子α对小鼠骨髓型放射损伤的防护作用 |
| Radioprotective effects of tumor necrosis factor-α on murine bone marrow damage |
| 投稿时间：1999-09-20 |
| DOI： |
| 中文关键词: 肿瘤坏死因子 造血干细胞 放射防护 |
| 英文关键词:Tumor necrosis factor α (TNFα) Hematopoietic progenitor cells (HPCs) Radioprotection |
| 基金项目:核工业总公司科研基金资助项目(CNNC94Q62059) |
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| 中文摘要: |
| 目的 研究肿瘤坏死因子α(TNFα)对受照射小鼠骨髓造血细胞的近期影响和造血重建功能的作用, 探讨TNFα对骨髓造血细胞的辐射防护作用。方法 小鼠照射前24h一次性注射TNFα,2周后检测其外周血WBC、骨髓MNC、CFU-S和CFU-Mix, 流式细胞仪分析骨髓cKit+细胞;并进行长期造血重建实验。结果 辐照前注射TNFα的小鼠, 2周后外周血WBC、骨髓MNC、内源性CFU-S、CUF-Mix数量和cKit+细胞百分数均显着高于未注射TNFα的辐照小鼠, 生存期亦延长;接受TNFα处理的小鼠骨髓细胞移植到受致死性照射的受体鼠后, 多具有良好的造血重建功能。结论 照射前给予TNFα对造血干细胞具有辐射保护作用。 |
| 英文摘要: |
| Objective To investigate the effects of tumor necrosis factor α(TNFα)on the bone marrow (BM)hematopoietic progenitor cells (HPCs)in irradiated mice and the radioprotection of TNFα on BM HPCs.Methods TNFα was injected into the mice before irradiation. Peripheral WBC, BMmononuclear cells (MNCs), CFU-S, CFU-Mix and hematopoietic reconstitution ability were examined on lethally irradiated mice with or without TNFα pre-treatment. Surface marker and c-Kit+ of BMstem cells was analyzed with flow cytometry. Results The mice with TNF α treatment were markedly higher than those without TNFα treatment in number of WBC, BM MNCs, CFU-S, and CFU-Mix in vitro and in the percentage of BMc-Kit+ cells. The life span of the former was longer than that of the latter. In hematopoietic reconstitution, the above criteria of the recipient mice given the BMcells derived from donor mice of pre-irradiation TNFα treatment were remarkable superior to those given the donor without TNFα treatment.Conclusion Pre irradiation treatment has obviously radioprotective effect on the BM HPCs. The experiment provides a novel approach to lessening BMhematopoietic arrest caused by chemothrapy and radiotherapy in tumor patients, and is of great significance in clinical application. |
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