| 徐永忠,郑斯英,赵经涌,曹建平,朱巍.双色荧光原位杂交检测60Coγ射线诱发染色体畸变的剂量效应关系[J].中华放射医学与防护杂志,2000,20(4):254-256 |
| 双色荧光原位杂交检测60Coγ射线诱发染色体畸变的剂量效应关系 |
| Dose response curves for chromosome aberrations induced by 60Coγ-rays and analysed by dual colour fluorescence in situ hybridization |
| 投稿时间：1999-10-30 |
| DOI： |
| 中文关键词: 荧光原位杂交 易位 双着丝粒体 剂量效应关系 |
| 英文关键词:Fluorescence in situ hybridization Translocation Dicentric Dose response relationship |
| 基金项目:国防科工委基金资助项目(Y5573262) |
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| 中文摘要: |
| 目的 探索荧光原位杂交(FISH)技术检测稳定性染色体畸变(易位)作为辐射生物剂量计的可行性。方法 用1号、4号全染色体组合探针的FISH技术及常规法检测不同剂量60Coγ射线诱发的染色体易位率和双着丝粒体率并拟合剂量效应曲线。结果 FISH技术检出的易位率、双着丝粒体率及常规法检出的双着丝粒体率与剂量间均可拟合成线性平方方程Y=c+αD+βD2.FISH技术和常规法检出的双着丝粒体率差异无显着性(P>0.05).对于1号和4号染色体, 辐射诱发的易位率、双着丝粒体率的观察值与基于DNA含量的预期值相比, 差异皆无显着性。结论 荧光原位杂交技术能快速、准确地检测易位且有良好的量效关系, 因此, 可望成为估算慢性受照者的累积剂量和早先受照者剂量重建的理想方法。 |
| 英文摘要: |
| Objective To explore the feasibility of using fluorescence in situ hybridization (FISH) to detect stable chromosome translocation as a biological dosimeter.Methods Translocation and dicentric frequencies in peripheral blood lymphocytes induced by 60Co γ-rays at different doses were analysed by conventional staining and dual colour FISHwith composite whole chromosome specific probes for human chromosomes 1 and 4, and their dose response curves were fitted. Results All dose response curves for translocation and dicentrics from FISHanalysis, as well as dicentrics from conventional staining could be described by linear quadratic equations Y=c+αD+βD2. The difference between dicentric frequencies analysed by FISHand conventional staining were not statistically significant ( P >0.05). For radiation induced translocation and dicentrics in chromosomes 1 and 4, no significant differences were found between the observed frequencies and expected frequencies based on DNAcontent.Conclusion Chromosome translocation can be quickly and accurately analysed by FISHand has a good dose response relationship; thus, it is hopeful to use translocation frequencies measured by FISHas a long term or accumulative biological dosimeter. |
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