| 高临路,崔玉芳,杨红,夏国伟,彭瑞云,高亚兵,王德文.Fas和Bcl2在受照射小鼠脾淋巴细胞中的表达及与细胞凋亡的关系[J].中华放射医学与防护杂志,2000,20(4):252-253 |
| Fas和Bcl2在受照射小鼠脾淋巴细胞中的表达及与细胞凋亡的关系 |
| Expression of Fas and Bcl-2 and their relationship to apoptosis in spleen lymphocytes of mice irradiated with large dose 60Coγ-rays |
| 投稿时间：1999-09-18 |
| DOI： |
| 中文关键词: 照射 脾淋巴细胞 凋亡 Fas Bcl-2 |
| 英文关键词:Irradiation Spleen lymphocytes Apoptosis Fas Bcl-2 |
| 基金项目:解放军总后勤部留学回国人员启动基金资助项目(9405021);军事医学科学院第二届创新基金资助项目(9805106) |
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| 中文摘要: |
| 目的 探讨Fas和Bcl2蛋白在受大剂量60Coγ射线照射后小鼠脾淋巴细胞中的表达及与细胞凋亡的关系。方法 小鼠全身一次性γ射线照射, 吸收剂量分别为0、6、9、12、15和20Gy, 于照射后3、6、12、24h、3、7、14和28d活杀取材, 免疫组化LSAB染色观察后进行定量分析。结果 照射后6h, Fas的表达呈强阳性, 6～12Gy剂量范围内阳性表达更明显, 而随时间递增表达逐渐减少, 但无明显的剂量效应关系, 7d后表达明显减弱。Bcl2的表达于6h几乎为阴性, 在<12Gy剂量组更为明显。至照射后28d依然未恢复。结论 在受大剂量照射小鼠脾淋巴细胞中Fas癌基因蛋白表达升高与细胞凋亡有较好相关关系, 即Fas蛋白可能有促进凋亡的作用。而在相同剂量照射后, Bcl2抑制凋亡的作用被减弱甚至消失。上述两种癌蛋白对受大剂量60Coγ射线照射的小鼠脾淋巴细胞凋亡均具有重要调控作用。 |
| 英文摘要: |
| Objective To investigate the expressions of Fas and Bcl-2 and their significance in apoptosis of spleen lymphocyte of mice after large dose γ-ray irradiation.Methods At 3, 6, 12, 24 h, 3, 7, 14 and 28 d after 6-20 Gy γ-ray irradiation mice were sacrificed and their spleens were removed.The expressions of Fas and Bcl-2 oncoprotein were analysed by LSABimmunohistochemical method. Results The expression of Fas was strongly positive at 6 h after irradiation, especially in 6-12 Gy groups. It became less obvious along with prolongation of time after irradiation and almost disappeared on d 7 after irradiation. The expression of Bcl-2 was nearly negative at 6 h after irradiation, especially in 12-20 Gy groups, and did not recover on d 28 after irradiation.Conclusion After large dose γ-ray irradiation the expression of Fas in mouse spleen lymphocytes shows a better relationship to lymphocyte apoptosis; in other words, Fas can prompt apoptosis. On the other hand, the action of Bcl-2 is reduced or even disappeared. Both of them play an important role in spleen lymphocyte apoptosis after large dose of γ-irradiation. |
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