| 朱茂祥,杨陟华,龚诒芬,徐勇.60Coγ射线对BEP2D细胞氧化损伤的研究[J].中华放射医学与防护杂志,2000,20(4):248-251 |
| 60Coγ射线对BEP2D细胞氧化损伤的研究 |
| Oxidative damage of BEP2D cells irradiated 60Coγ-rays |
| 投稿时间：1999-09-26 |
| DOI： |
| 中文关键词: 60Coγ射线照射 BEP2D细胞 活性氧 8羟基脱氧鸟嘌呤 硒 |
| 英文关键词:60Co γ-ray irradiation HPV-16 immortalized human bronchial epithelial cell Reactive oxygen species 8-Hydroxydeoxygunosine Selenium |
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| 摘要点击次数: 2599 |
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| 中文摘要: |
| 目的 研究60Coγ射线照射人乳头状病毒永生化的人支气管上皮细胞(BEP2D)产生的活性氧(ROS)及其对DNA的氧化损伤, 以及纳米硒的保护作用。方法 细胞上清中过氧化氢(H2O2)、超氧阴离子(O2)以及羟自由基(·OH)含量分别用辣根过氧化物酶介导的酚红氧化法、细胞色素C还原法和番红花红退色法进行测定;细胞内H2O2和O2分别用2', 7'二氯荧光黄双乙酸盐(DCFHDA)和氢化乙锭(HE)标记, 用流式细胞法测定荧光产物2', 7'二氯荧光黄(DCF)和溴乙锭(EB)荧光强度;8羟基脱氧鸟嘌呤(OH8dG)含量用高压液相色谱结合电化学方法(HPLCECD)测定。结果 BEP2D细胞经不同剂量的60Coγ射线照射后, 细胞内及细胞上清中产生的ROS以及OH8dG含量均显着增加, 并有较好的剂量效应关系。1μmol/L的纳米硒能明显减少60Coγ射线照射后BEP2D细胞产生的ROS及OH8dG水平。结论 60Coγ射线照射能造成细胞的氧化损伤, 纳米硒对60Coγ射线照射所致细胞的氧化损伤有保护作用。 |
| 英文摘要: |
| Objective To study reactive oxygen species (ROS) productions and oxidative DNAdamage in HPV-16 immortalized human bronchial epithelial cells(BEP2D) irradiated with 60Co γ-rays. Methods The measurement of extracellular superoxide anions (O 2) was based on the reduction of ferricytochrome Cas assayed by the increase in its absorbance at 550 nm.Quantitation of extracellular hydrogen peroxides (H2O2) was based on the horseradish peroxidase-dependent oxidation of phenol red which is assayed by its increased absorbance at 620 nm.The determination of extracellular hydroxyl radicals (·OH) was based on decoloration of safranine Tas assayed by the decrease in its absorbance at 520 nm.Ethidium bromide and 2’, 7’-dichlorofluorescein, fluorescent products of the membrane-permeable dyes-hydroethine and 2’, 7’-dichloroflurescin diacetate, were used to monitor the intracellular production of H2O2 and O 2 respectively by flow cytometry.8-hydroxydeoxygunosine (OH8dG) was examined with HPLC-ECDfrom extracted DNA. Results The ROSproduction including H2O2, O2 and ·OH, and DNAadduct OH8dGin60 Co γ-rays-irradiated BEP2D cells increased remarkably, and these increments could be inhibited effectively by 1 μmol/Lof selenium. Conclusion 60Co γ-irradiation causes oxidative damage to BEP2D cells, which can be protected by selenium. |
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