| 张占英,诸雪珍,张澜生,朱寿彭.脐血移植中移植物抗宿主病低发生机理研究[J].中华放射医学与防护杂志,2000,20(4):245-247 |
| 脐血移植中移植物抗宿主病低发生机理研究 |
| Mechanism of low GVHD incidence in umbilical cord blood transplantation |
| 投稿时间：1999-10-19 |
| DOI： |
| 中文关键词: 脐血血清 T淋巴细胞 可溶性因子 GVHD |
| 英文关键词:Umbilical cord blood T cells Soluble factors Graft-versus-host disease |
| 基金项目::国家自然科学基金资助项目(39970244);国家核工业基金资助项目(Y7197Q6206) |
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| 中文摘要: |
| 目的 通过对脐血和外周血免疫功能的比较, 探讨了脐血移植中移植物抗宿主病(GVHD)低发生率的分子机理。方法 经增殖试验、NK杀伤活性试验及免疫荧光双标记方法研究了4种血清对T细胞免疫功能的影响。结果 与成人血清相比较, 脐血血清能降低NK细胞杀伤肿瘤靶细胞的活性;同时脐血血清和成人血清都能增加丝裂原特异性T淋巴细胞增殖, 成人血清在增殖反应中可达到峰值, 而脐血血清没有达到峰值效应, 甚至在0.1的稀释度时仍没有下降;成人血清能增加PHA刺激外周血单个核细胞(PBMC)中的T淋巴细胞HLADR表达, 而脐血血清却没有这种效应。结论 血清中所分泌的某些可溶性因子可能在T细胞活化中起重要作用, 与GVHD发生机理相关联。 |
| 英文摘要: |
| Objective To compare the immunological function between umbilical cord blood (UCB) and peripheral blood, and to explore the mechanism of low incidence of graft-versus host disease (GVHD) in UCBtransplantation. Methods The proliferation assay, NKcell killing activity assay and immunologic fluorescence double label technique were used to study four sear effect on Tcell immunologic function. Results UCBserum could reduce K562 cell activity, compared to that of adult serum.Furthermore, adult serum will enhanced the percentage of HLA DRexpression of Tcells on PHA-stimulated PBMC, whereas fetal serum has no such effect.Both adult and fetal serum enhanced mitogen-specific Tcell proliferation, the peak proliferative response could be reached with adult serum but not with fetal serum. Conclusion There must be some soluble factors secreted into serum, which play a role in Tcell activation and associate with the incidence of GVHD. |
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