| 江国春,袁丽珍,魏康,郭学敏,张雪峰,贾向旭.小鼠乳腺癌辐射敏感细胞SX-9的ku基因突变与辐射敏感性研究[J].中华放射医学与防护杂志,2000,20(4):242-244 |
| 小鼠乳腺癌辐射敏感细胞SX-9的ku基因突变与辐射敏感性研究 |
| ku gene mutation of radiosensitive mouse mammary carcinoma derived cell line SX-9 |
| 投稿时间：1999-09-28 |
| DOI： |
| 中文关键词: 辐射敏感性 ku基因 基因突变 |
| 英文关键词:Radiosensitivity ku gene Gene mutation |
| 基金项目:国家自然科学基金资助项目(39900031) |
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| 中文摘要: |
| 目的 研究辐射敏感小鼠乳腺癌细胞SX-9的ku70、ku80基因型, 初步探讨ku基因突变对其辐射敏感性的影响。方法 RTPCR克隆并鉴定ku70/ku80全长基因, 并对其全长进行全自动测序。脂质体转染正常人全长ku80cDNA至SX-9细胞, 克隆形成比较转染了正常ku80基因的SX-9与SX-9以及SR1细胞存活率。结果 SX-9细胞ku70基因正常, 而其ku80存在基因突变, 转染了正常人全长ku80cDNA的SX-9细胞能部分重建其对γ射线的存活率。结论 SX-9细胞ku基因发生突变, 这可能是其DNA损伤修复缺陷并导致对辐射敏感的分子基础。 |
| 英文摘要: |
| Objective To investigate the ku gene status of radiosensitive cell line SX-9 and the effects of ku80 gene mutation on radiosensitivity of SX-9. Methods After comparing the ku70/80 gene status of SX-9 to SR-1 with molecular cloning, the survival fraction of SX-9 transfected with wild-type human ku80, SX-9 and SR-1 was investigated by clonogenic assay. Results Radiosensitive mouse mammary carcinoma-derived cell line SX-9 bore mutation in ku80 gene, and its survival fraction after γ-ray irradiation could be restored by transfection of wild-type human ku80 gene. Conclusions There are two mutation sites in ku80 gene in radiosensitive mouse mammary carcinoma-derived cell line SX-9, which could be the cause of its deficiency in repair of DNAdouble strand breaks. |
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