| 吴建秋,杨云纺,金志军,蔡建明,杨如俊,项莺松.人脐血分次移植重症联合免疫缺陷小鼠的实验研究[J].中华放射医学与防护杂志,2000,20(4):239-241 |
| 人脐血分次移植重症联合免疫缺陷小鼠的实验研究 |
| Scheduled transplantation of human umbilical cord blood to severe combined immunodificient mice |
| 投稿时间：1999-09-06 |
| DOI： |
| 中文关键词: 脐血 造血干细胞 重症联合免疫缺陷小鼠 分次移植 |
| 英文关键词:Umbilical cord blood Hematopoietic stem cells SCID mice Scheduled transplantation |
| 基金项目:国家自然科学基金资助项目(39600077) |
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| 摘要点击次数: 2924 |
| 全文下载次数: 2020 |
| 中文摘要: |
| 目的 探讨提高脐血移植效果的新方法, 进一步了解脐血造血干细胞体内生长特性。方法 以经亚致死剂量γ射线照射后的重症联合免疫缺陷小鼠为动物模型, 进行冻存人脐血的分次移植。利用人粒单系祖细胞培养及流式细胞仪, 检测植入小鼠骨髓等器官中人源性细胞。结果 与一次移植相比, 分次移植相同细胞数时, 小鼠骨髓中人CD45+、CD34+细胞水平明显提高;分次移植细胞数降低到20%时, 其造血及部分免疫功能恢复到相近水平。结论 脐血分次移植提高了干细胞的植入率, 为临床成人脐血移植提供了一条新思路。 |
| 英文摘要: |
| Objective To explore a new method for developing the efficiency of human umbilical cord blood (UCB) cells engraftment, and further understand the growth characteristic of hematopoietic stem cells (HSC) in vivo . Methods Sublethally irradiated severe combined immunodeficient (SCID) mice were transplanted i.v.with UCBcells which had been cryopreserved at -80℃.The human cells in recipient mice were detected by flow cytometry and CFU-GMassay. Results In contrast to the single transplantation, scheduled engraftment of similar numbers of UCBcells resulted in a proportionally obvious increase in the percentages of CD45+, CD34+ cells produced in SCIDmouse bone marrow (BM).When the donor cells were reduced to 20 percent, an identical reconstitution of both hematopoietic and part of immunologic functions was achieved. Conclusion Scheduled engraftment improves the repopulating ability of HSC, which would provide a novel way for clinical cord blood engraftment in adult objects. |
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